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| Status |
Public on Aug 27, 2012 |
| Title |
Genome-wide Transcriptional Responses of the Filamentous Fungus Aspergillus niger to Lignocellulose (Wheat straw) using RNA-sequencing |
| Organism |
Aspergillus niger |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
We have studied the physiological response of the fungus Aspergillus niger when exposed to wheat straw as a model lignocellulosic substrate. Using RNA-sequencing we showed that, 24 hours after exposure to straw, gene expression of known plant cell wall degrading enzymes represents a huge investment for the cells (about 20 % of the total mRNA). Our results also uncovered new esterases and surface interacting proteins that might form part of the fungal degradative arsenal. We also show that antisense transcripts are abundant and that their expression can be regulated by conditions.
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| Overall design |
Triplicate samples of A. niger N402 taken at each of three timepoints: After 48 h growth in minimal media with Glucose as sole carbon source, After transfer to Wheat Straw media for 24 h and 5 h after after the exogenous addition of glucose to the wheat starw media.
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| Contributor(s) |
Delmas S, Pullan ST, Gaddipati S, Kokolski M, Malla S, Blythe MJ, Ibbet R, Campbell M, Liddell S, Aboobaker A, Tucker GA, Archer DB |
| Citation(s) |
22912594, 24792495, 28955445 |
| |
| Submission date |
Nov 21, 2011 |
| Last update date |
Jul 10, 2019 |
| Contact name |
David Archer |
| E-mail(s) |
plzstp@nottingham.ac.uk, david.archer@nottingham.ac.uk
|
| Organization name |
University of Nottingham
|
| Department |
School of Biology
|
| Lab |
Archer Lab
|
| Street address |
University Park
|
| City |
Nottingham |
| ZIP/Postal code |
NG7 2RD UK |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL14910 |
AB SOLiD System 3.0 (Aspergillus niger) |
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| Samples (9)
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| Relations |
| SRA |
SRP009412 |
| BioProject |
PRJNA148183 |
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