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Series GSE33812 Query DataSets for GSE33812
Status Public on Nov 19, 2011
Title Gene expression profiles of bone marrow mesenchymal stem cells in pediatric patients with severe aplastic anemia
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Pediatric severe aplastic anemia (PSAA) is a rare and life-threatening disease, which has been suggested to result from deficiency in essential cytokines or growth factors in bone marrow mesenchymal stem cells (MSCs). In this study, we examined expression profiles of the essential cytokines and growth factors, which were involved in proliferation and differentiation of MSC, in 5 PSAA patients using oligonucleotides microarrays. Following evaluation of gene expression patterns using cluster analysis and gene ontology classifications, fifteen potential genes that were cell proliferation- and cytokine-related and significantly down-regulated in PSAA were selected. The findings of the microarray analysis were validated by quantitative reverse transcription-polymerase chain reaction (qRT-PCR). Interestingly, silencing of chemokine ligand 12 (CXCL12, also named stromal cell-derived factor 1, SDF1) or hepatocyte growth factor (HGF) gene expression reduced cell growth and response to adipogenic factor-induced differentiation, but increased osteogenesis, which possibly derived from hematopoietic stem cells (HSCs). Re-introduction of the CXCL12 gene or exogenously added HGF, on the other hand, partially recovered cell function of PSAA MSC. These results suggest that gene expression in PSAA MSC is globally down-regulated by a yet-to-be-determined mechanism. Among these down-regulated genes, CXCL12 and HGF played vital roles in regulating cell growth and differentiation.
Overall design Mesenchymal stem cells from five pediatric SAA patients vs. MSC from five healthy donors, which were pooled together.
Contributor(s) Chow K
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Submission date Nov 18, 2011
Last update date Feb 22, 2018
Contact name Kuan-Chih Chow
Phone +886-4-22840896-118
Fax +886-4-22859270
Organization name National Chung Hsing University
Department Graduate Institute of Biomedical Sciences
Lab Cancer Research Lab.
Street address 250 Kuo Kuang Road
City Taichung
ZIP/Postal code 40227
Country Taiwan
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (5)
GSM836205 MSC from patient B240
GSM836206 MSC from patient B256
GSM836207 MSC from patient B226
BioProject PRJNA148137

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33812_RAW.tar 45.2 Mb (http)(custom) TAR (of TXT)
GSE33812_sorted_Mvalues.txt.gz 12.2 Kb (ftp)(http) TXT
Processed data included within Sample table
Processed data are available on Series record

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