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Series GSE33755 Query DataSets for GSE33755
Status Public on Jun 22, 2012
Title Effect of IL-1β on mesenchymal stem cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Mesenchymal stem cells are often transplanted in inflammatory environments and they are able to survive and modulate host immune responses through mechanism poorly understood. In this paper we analyzed biological responses of MSC in response IL-1β as a representative inflammatory mediator. Microarray analysis of MSC treated with IL-1β revealed that this cytokine activates a set of biological process related with cell survival, cell migration, cell adhesion, chemokine production, induction of angiogenesis and modulation of immune response. Further analysis by real-time PCR and functional assays revealed that IL-1β mainly increases production of chemokines CCL5, CCL20, CXCL1, CXCL3, CXCL5, CXCL6, CXCL10, CXCL11 and CX3CL1, interleukins IL-6, IL-8, IL23A, IL32, Toll-like receptors TLR2, TLR4, CLDN1, metalloproteins MMP1 and MMP3, growth factors CSF2 and TNF, together with adhesion molecules ICAM1 and ICAM4. Functional analysis of MSC proliferation, migration and adhesion to extracellular matrix components revealed that IL-1β did not affect proliferation whereas constitute a throphic factor for MSC and increases adhesion mainly to collagen and laminin. Moreover, IL-1β treatment enhanced the ability of MSC to recruit monocytes and granulocytes cells in vitro. Blockade of NFkβ transcription factor activation with IκB kinase beta (IKKb) siRNA impaired MSC migration, adhesion and leucocyte recruitment, indicating that NF-kB signaling pathway is the main mechanism implicated in these biological processes. These findings are relevant for designing protocols that implicate MSC delivery into inflammatory environments.
Overall design Comparison between two experimental conditions, one sample for each condition
Contributor(s) Rubén C, Inmaculada C, Elisa L, Joaquín D, Francisco G, Paz RM, César T, Akaitz D, Amparo R, Anastasio MJ, Pilar S
Citation(s) 22467443
Submission date Nov 16, 2011
Last update date Feb 22, 2018
Contact name Francisco García García
Organization name CIPF
Department Bionformatics & Biostatistics Unit
Street address Eduardo Primo Yúfera, 3
City Valencia
ZIP/Postal code 46012
Country Spain
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (2)
GSM834993 MSC
GSM834994 MSC_IL
BioProject PRJNA148405

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33755_RAW.tar 24.8 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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