GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE33701 Query DataSets for GSE33701
Status Public on Jan 25, 2012
Title Increased sensitivity of next generation sequencing-based expression profiling after globin reduction in human blood RNA
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary Transcriptome analysis is of great interest in clinical research, where significant differences between individuals can be translated into biomarkers of disease. Although next generation sequencing provides robust, comparable and highly informative expression profiling data, with several million of tags per blood sample, reticulocyte globin transcripts can constitute up to 76% of total mRNA compromising the detection of low abundant transcripts. We have removed globin transcripts from 6 human whole blood RNA samples with a human globin reduction kit and compared them with the same non-reduced samples using deep Serial Analysis of Gene Expression.
Overall design Comparison of 6 whole blood RNA samples versus their globin reduced counterparts
Contributor(s) Mastrokolias A, den Dunnen JT, van Ommen GJ, ’t Hoen PA, van Roon-Mom WM
Citation(s) 22257641
Submission date Nov 15, 2011
Last update date May 15, 2019
Contact name Anastasios Mastrokolias
Phone 0031715269425
Organization name Leiden University Medical Center
Street address LUMC, Building 2 Einthovenweg 20 2333 ZC Leiden
City Leiden
ZIP/Postal code 2333 ZC
Country Netherlands
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (12)
GSM833103 Sample1
GSM833104 Sample1Reduced
GSM833105 Sample2
SRA SRP009329
BioProject PRJNA148285

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33701_count_data.txt.gz 379.8 Kb (ftp)(http) TXT
SRA Run SelectorHelp
Raw data are available in SRA
Processed data are available on Series record

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap