GEO Logo
   NCBI > GEO > Accession DisplayHelp Not logged in | LoginHelp
GEO help: Mouse over screen elements for information.
Series GSE33647 Query DataSets for GSE33647
Status Public on Jan 16, 2012
Title Early Peroxisome proliferator-activated receptor gamma regulated genes involved in expansion of pancreatic beta cell mass
Organism Mus musculus
Experiment type Expression profiling by array
Summary The progression towards type 2 diabetes depends on the success of the allostatic response of the pancreatic beta cells to synthesise and secrete enough insulin to compensate for insulin resistance. The endocrine pancreas is a plastic tissue able to expand or regress in response to the requirements imposed by physio and pathological states such as pregnancy, obesity or ageing. The mechanisms, mediating beta cell mass expansion in these scenarios are not well defined. We have recently showed that beta cell mass failed to expand in ob/ob mice with genetic ablation of PPARγ2, a mouse model known as the POKO mouse.
This phenotype contrasted with the appropriate expansion of the beta cell mass observed in their obese littermate ob/ob mice. Here we investigate PPAR gamma dependent transcriptional responses occurring during early stages of the adaptation of beta cells to insulin resistance. As it could be expected we have identified genes known to regulate proliferation and survival signals of the beta cells. Moreover we have also identified new pathways induced in ob/ob islets that fail to do so in POKO islets. Our data suggest that the expansion of beta cell mass observed in ob/ob islets is associated with activation of immune response and is missing in POKO islets. Other PPARγ dependent differentially regulated pathways include cholesterol biosynthesis, apoptosis through TGF-β signaling and decreased oxidative phosphorylation.
Overall design In this study, we used gene expression arrays to investigate differences between four experimental classes by pairs
Contributor(s) Vivas Y, Martínez-García C, Izquierdo A, García-García F, Callejas S, Velasco I, Campbell M, Ros M, Dopazo A, Dopazo J, Vidal-Puig A, Medina-Gomez G
Citation(s) 22208362
Submission date Nov 12, 2011
Last update date Jan 12, 2017
Contact name Francisco García García
Organization name CIPF
Department Bionformatics & Biostatistics Unit
Street address Eduardo Primo Yúfera, 3
City Valencia
ZIP/Postal code 46012
Country Spain
Platforms (1)
GPL7202 Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Probe Name version)
Samples (12)
GSM832101 DKO rep1
GSM832102 DKO rep2
GSM832103 DKO rep3
BioProject PRJNA148653

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE33647_RAW.tar 22.5 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

| NLM | NIH | GEO Help | Disclaimer | Accessibility |
NCBI Home NCBI Search NCBI SiteMap