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| Status |
Public on Nov 02, 2011 |
| Title |
Discovery of novel microRNAs in rat kidney using microarray analyses |
| Platform organisms |
Petromyzon marinus; Danio rerio; Oryzias latipes; Xenopus laevis; Xenopus tropicalis; Gallus gallus; Ornithorhynchus anatinus; Ateles geoffroyi; Macaca mulatta; Macaca nemestrina; Symphalangus syndactylus; Pan paniscus; Pan troglodytes; Pongo pygmaeus; Homo sapiens; Canis lupus familiaris; Equus caballus; Sus scrofa; Bos taurus; Ovis aries; Mus musculus; Rattus norvegicus; Takifugu rubripes; Taeniopygia guttata; Rhinopithecus bieti; Saguinus labiatus; Tetraodon nigroviridis |
| Sample organism |
Rattus norvegicus |
| Experiment type |
Non-coding RNA profiling by array
|
| Summary |
MicroRNAs are small non-coding RNAs that regulate a variety of biological processes. In the last version of the miRBase database (Release 17), 720 mouse microRNAs are accompanied by only 408 rat microRNAs. Given the importance of rat as a model organism, we used next generation sequencing and microarray technologies to discover novel microRNAs in rat kidneys.
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| Overall design |
Four male, 6-week-old Big Blue rats were treated with 10.0 mg/kg aristolochic acid(AA) 5 times a week for 12 weeks, four untreated rats as control. The animals were sacrificed 1 day after the last treatment and total RNA were isolated. All eight rat samples were used to deep sequencing analyses (University of Texas Southwestern Medical Center Microarray Core Facility), while 6 samples (3 AA-treated and 3 control) were used to custom vertebrate microRNA microarray analysis (LC Sciences). We used the miRanalyzer standalone version for the prediction of novel microRNAs and microRNA microarray to confirm novel microRNAs.
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| Contributor(s) |
Meng F, Hackenberg M, Li Z, Yan J, Chen T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Nov 01, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Fanxue Meng |
| E-mail(s) |
fanxue.meng@fda.hhs.gov
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| Phone |
870-543-7082
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| Organization name |
National Center for Toxicological Research
|
| Street address |
3900 NCTR Road, HFT-130
|
| City |
Jefferson |
| State/province |
AR |
| ZIP/Postal code |
72079 |
| Country |
USA |
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| Platforms (1) |
| GPL14819 |
LC Sciences_Vertebrate_microRNA_Version17 (MRA-1033) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA149019 |
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