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| Status |
Public on Oct 22, 2011 |
| Title |
The redox-sensitive transcription factor, Nrf2, regulates murine hematopoietic stem cell survival |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
To better understand the differentiation and survival defect of Nrf2-/- HSPC, we performed gene expression studies on KSL cells isolated by FACS from Nrf2+/+ and Nrf2-/- mice.
Gene expression data suggests that loss of Nrf2 leads to global defects in cytokine signaling, and the administration of the exogenous granulocyte colony-stimulating factor (G-CSF) improved HSPC survival despite increasing intracellular ROS.
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| Overall design |
Bone marrow cells from young healthy Nrf2+/+ and Nrf2-/- mice (n=5 per genotype ) were pooled, stained with KSL cell marker and FACS. Total RNA was isolated from the pooled KSL cells, quantified, amplified and profiled. One pooled RNA sample from each genotype (Nrf2+/+ and Nrf2-/-) was profiled using Affymetrix GeneChip Mouse Gene ST 1.0 chips.
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| Contributor(s) |
Merchant AA, Singh A, Matsui W, Biswal S |
| Citation(s) |
22039262 |
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| Submission date |
Oct 21, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Biswal Shyam |
| E-mail(s) |
sbiswal@jhsph.edu
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| Organization name |
Johns Hopkins University
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| Department |
EHS
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| Lab |
E7210
|
| Street address |
615 N Wolfe Street
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| City |
Baltimore |
| State/province |
MD |
| ZIP/Postal code |
21205 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (2) |
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| Relations |
| BioProject |
PRJNA149385 |
