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Series GSE3306 Query DataSets for GSE3306
Status Public on Sep 21, 2005
Title Gene induction during differentiation of human pulmonary type II cells in vitro
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Studies of human fetal lung in explant culture and in isolated epithelial cells have demonstrated that both glucocorticoids and cyclic AMP promote differentiated alveolar type II cell phenotype as assessed by ultrastructural morphology and surfactant production. This project profiles changes in gene expression associated with hormone induced differentiation. Undifferentiated human fetal lung (13-20 wk) epithelial cells were cultured in serum-free medium (control) or with dexamethasone/8-Bromo cyclic AMP/isobutylmethylxanthine (DCI) to promote type II cell differentiation. RNA from five sets of experiments (10 samples) was evaluated using the U133A Affymetrix GeneChip set.
Keywords: Hormone treatment
Overall design Cells were isolated from 13 individual lungs and the cells from each were cultured in the absence (control) and presence of DCI dexamethasone (10 nM)/8-Br-cyclic AMP (0.1 mM)/isobutylxanthine for 72 h. RNA was isolated from the control and treated cells of each prep. For the 5 sets of microarray experiments (10 total chips, 2 for each control and treated pair), RNA from 2 of the cell preps was analysed as individual microarray comparisons. RNA from 11 cell preps was pooled for the other 3 experiments (4, 4, and 3 cell preps) to provide control and DCI-treated RNA pools.
Contributor(s) Wade K, Guttentag SH, Gonzales LW, Maschhoff K, Gonzales J, Kolla V, Ballard PL
Citation(s) 16474099, 16997883
Submission date Sep 14, 2005
Last update date Aug 10, 2018
Contact name Philip L Ballard
Phone 415 476-4193
Organization name UCSF
Department Neonatology
Lab HSE 1421
Street address 513 Parnassus St
City San Francisco
State/province CA
ZIP/Postal code 94194
Country USA
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (10)
GSM74250 Control 1, 72 h
GSM74263 Treated 1, 72 h
GSM74266 Control 2, 72 h
BioProject PRJNA92821

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