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Series GSE32916 Query DataSets for GSE32916
Status Public on May 03, 2012
Title Analysis of Pro-Inflammatory Gene Activation and RNA Processing by RNAseq of Nascent Transcripts I
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary We examined pro-inflammatory gene activation in activated murine macrophages by performing RNA-Seq with fractionated chromatin-associated, nucleoplasmic, and cytoplasmic transcripts. This experimental strategy allowed a global, high-resolution analysis of the transcriptional regulation of diverse classes of co-expressed genes, with a direct comparison to transcript processing and the transit of RNA from the chromatin to the nucleoplasm and the cytoplasm. The results provide quantitative insights into the relationship between transcription and distinct promoter and chromatin properties, as well as genome-scale insights into RNA processing and dynamics.
Overall design RNA-seq of subcelluar fractions from five stimulation time-points
Contributor(s) Smale ST, Bhatt DM, Tong A
Citation(s) 22817891, 23616639
Submission date Oct 12, 2011
Last update date Jan 25, 2024
Contact name Allison Daly
Organization name UCLA
Department MIMG
Lab Smale Lab
Street address 6-730 MRL 675 Charles E. Young Drive South
City Los Angeles
State/province Ca
ZIP/Postal code 90024
Country USA
Platforms (1)
GPL13112 Illumina HiSeq 2000 (Mus musculus)
Samples (15)
GSM814832 Cytoplasmic_0min
GSM814833 Cytoplasmic_15min
GSM814834 Cytoplasmic_30min
SRA SRP008831
BioProject PRJNA146475

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32916_RAW.tar 16.9 Gb (http)(custom) TAR (of BAM)
GSE32916_classes_rpkm.xls.gz 1.8 Mb (ftp)(http) XLS
GSE32916_readme.txt 158 b (ftp)(http) TXT
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Processed data provided as supplementary file
Raw data are available in SRA

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