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| Status |
Public on Oct 13, 2011 |
| Title |
Oxidative damage and respiratory burst in Multiple Sclerosis |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In this project we focused on white matter injury in relapsing remitting MS. We concentrated on material obtained from patients who suffered from fulminant active MS to identify possible sources for ROS production in relation to demyelination and neurodegeneration.
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| Overall design |
We performed genome-wide microarray analyses of gene expression in carefully dissected lesions of patients with fulminant acute MS. Preceding the gene expression profiling all cases were characterized histologically and areas of interest were identified. We included 4 control cases, 2 active MS cases from which we separated 3 distinct lesions areas per case (NAWM, initial lesion and early demyelinated lesion) and 1 active MS case from which we separated 2 distinct lesion areas (NAWM and early demyelinated lesion). The RNA was isolated from those areas, amplified and hybridized to Agilent G4112A whole genome microarrays.
UPDATE in 2013! We additionally included a fourth acute MS case, from which we microdissected 2 different initial lesions and 2 different early demyelinated lesions. Data was re-normalized and re-analyzed.
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| Contributor(s) |
Fischer M, Sharma R, Wimmer I |
| Citation(s) |
22366799, 23868451, 24622774, 26087903, 28541408 |
| |
| Submission date |
Oct 12, 2011 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Isabella Wimmer |
| E-mail(s) |
isabella.wimmer@meduniwien.ac.at
|
| Phone |
+4314016034222
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| Organization name |
Medical University of Vienna
|
| Department |
Center for Brain Research
|
| Lab |
Department of Neuroimmunology
|
| Street address |
Spitalgasse 4
|
| City |
Vienna |
| ZIP/Postal code |
1090 |
| Country |
Austria |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA146545 |
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