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Series GSE32575 Query DataSets for GSE32575
Status Public on May 24, 2012
Title Transcriptome analysis of circulating monocytes in obese patients before and three months after bariatric surgery
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Low-grade chronic inflammation plays an important role in the development of obesity and obesity-associated disorders such as insulin resistance, type 2 diabetes, the metabolic syndrome and atherosclerosis. One possible link between obesity and inflammation is the enhanced activation of circulating monocytes making them more prone to infiltration into the adipose and vascular tissues of obese persons. Furthermore, weight loss after bariatric surgery is associated with less inflammation. Transcriptome analysis of circulating monocytes from control and obese patients before and after bariatric surgery will potentially provide insights into the pathophysiology of obesity and associated disorders and supply biomarkers for diagnostic purpose. The cohort comprised 6 lean age-matched controls (BMI: 20.3±0.5 kg/m2, mean±SEM) and 18 obese individuals without clinical symptoms of cardiovascular disease (BMI: 45.1±1.4 kg/m2, P<0.001 compared with lean controls). These 18 morbidly obese subjects were referred to our hospital for bariatric surgery. Before they were included, individuals were evaluated by an endocrinologist, an abdominal surgeon, a psychologist and a dietician. Only after multidisciplinary deliberation, the selected patients received a laparoscopic Roux-en-Y gastric bypass. CD14+ monocytes were collected before and three months after bariatric surgery (BMI: 37.5±1.3 kg/m2, P<0.001 compared with before weight loss), total RNA was extracted and subjected to genome-wide expression analysis.
 
Overall design Samples consisted of CD14+ monocytes from 6 lean controls and 18 morbidly obese patients before and three months after bariatric surgery. The 6 lean controls were also used to make 6 control pools.
 
Contributor(s) Geeraert B, Benhabilès N, Holvoet P
Citation(s) 22272346
Submission date Oct 03, 2011
Last update date Feb 15, 2013
Contact name Paul Holvoet
E-mail(s) paul.holvoet@med.kuleuven.be
URL http://www.kuleuven.be/amu
Organization name K.U.Leuven
Department Department of Cardiovascular Diseases
Lab Atherosclerosis and Metabolism Unit
Street address Herestraat 49, PB 705
City Leuven
ZIP/Postal code 3000
Country Belgium
 
Platforms (1)
GPL6102 Illumina human-6 v2.0 expression beadchip
Samples (48)
GSM807339 Monocyte_lean_rep1
GSM807340 Monocyte_lean_rep2
GSM807341 Monocyte_lean_rep3
Relations
BioProject PRJNA147093

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32575_RAW.tar 5.8 Mb (http)(custom) TAR
GSE32575_non-normalized.txt.gz 15.4 Mb (ftp)(http) TXT
Processed data included within Sample table
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