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| Status |
Public on May 08, 2012 |
| Title |
Dynamics of enhancer chromatin signatures mark the transition from pluripotency to cell specification during embryogenesis |
| Organism |
Danio rerio |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
The generation of distinctive cell types that form different tissues and organs requires precise, temporal and spatial control of gene expression. This depends on specific cis-regulatory elements distributed in the non-coding DNA surrounding their target genes. Studies performed on mammalian embryonic stem cells and Drosophila embryos suggest that active enhancers form part of a defined chromatin landscape marked by histone H3 lysine 4 mono-methylation (H3K4me1) and histone H3 lysine 27 acetylation (H3K27ac). Nevertheless, little is known about the dynamics and the potential roles of these marks during vertebrate embryogenesis. Here we provide genomic maps of H3K4me1/me3 and H3K27ac at four developmental time-points of zebrafish embryogenesis and analyze embryonic enhancer activity. We find that: (i) changes in H3K27ac enrichment at enhancers accompany the shift from pluripotency to tissue-specific gene expression; (ii) in early embryos, the peaks of H3K27ac enrichment are bound by pluripotent factors such as Nanog; (iii) the degree of evolutionary conservation is higher for enhancers that become marked by H3K27ac at the end of gastrulation suggesting their implication in the establishment of the most conserved (phylotypic) transcriptome that is known to occur later at the pharyngula stage.
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| Overall design |
ChIP-seq analysis of H3K4me3, H3K4me1 and H3K27ac at four developmental time-points (dome, 80% epiboly, 24hpf and 48pf) of zebrafish embryogenesis.
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| Contributor(s) |
Bogdanović O, Fernandez-Miñan A, Tena JJ, de la Calle-Mustienes E, Hidalgo C, van Heeringen SJ, Veenstra GJ, Gómez-Skarmeta JL |
| Citation(s) |
22593555 |
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| Submission date |
Sep 29, 2011 |
| Last update date |
May 15, 2019 |
| Contact name |
Ozren Bogdanovic |
| E-mail(s) |
o.bogdanovic@gmail.com
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| Organization name |
Garvan Institute of Medical Research
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| Department |
Genomics and Epigenetics
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| Street address |
384 Victoria Street
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| City |
Darlinghurst |
| State/province |
NSW |
| ZIP/Postal code |
2010 |
| Country |
Australia |
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| Platforms (1) |
| GPL10164 |
Illumina Genome Analyzer (Danio rerio) |
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| Samples (24)
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| GSM803825 |
H3K4me3_48hpf, danRer6 |
| GSM803826 |
H3K4me1_dome, danRer6 |
| GSM803827 |
H3K4me1_80%epi, danRer6 |
| GSM803828 |
H3K4me1_24hpf, danRer6 |
| GSM803829 |
H3K4me1_48hpf, danRer6 |
| GSM803830 |
H3K27ac_dome, danRer6 |
| GSM803831 |
H3K27ac_80%epi, danRer6 |
| GSM803832 |
H3K27ac_24hpf, danRer6 |
| GSM803833 |
H3K27ac_48hpf, danRer6 |
| GSM915189 |
H3K4me3_dome, danRer7 |
| GSM915190 |
H3K4me3_80%epi, danRer7 |
| GSM915191 |
H3K4me3_24hpf, danRer7 |
| GSM915192 |
H3K4me3_48hpf, danRer7 |
| GSM915193 |
H3K4me1_dome, danRer7 |
| GSM915194 |
H3K4me1_80%epi, danRer7 |
| GSM915195 |
H3K4me1_24hpf, danRer7 |
| GSM915196 |
H3K4me1_48hpf, danRer7 |
| GSM915197 |
H3K27ac_dome, danRer7 |
| GSM915198 |
H3K27ac_80%epi, danRer7 |
| GSM915199 |
H3K27ac_24hpf, danRer7 |
| GSM915200 |
H3K27ac_48hpf, danRer7 |
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| Relations |
| SRA |
SRP008826 |
| BioProject |
PRJNA147789 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE32483_RAW.tar |
2.0 Gb |
(http)(custom) |
TAR (of BED, WIG) |
SRA Run Selector |
| Processed data provided as supplementary file |
| Raw data are available in SRA |
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