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| Status |
Public on Feb 16, 2012 |
| Title |
Expression data from TOP-GFP sorted colon cancer cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Colon cancers typically contain tumor cell populations with differential WNT signaling activity. Colon cancer cells with high WNT-activity have been attributed increase tumorigenic potential and stem cell characteristics.
We extracted tumor cells with differential WNT activity using fluorescent reporters from xenografted human colon cancer cell line tumors and primary human colon cancer xenografts and analysed their gene expression profiles.
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| Overall design |
Colon cancer cell lines and primary colon cancer xenografts were transduced with lentiviral TOP-GFP reporters, and grown as xenografts in NOD-SCID mice. We disaggregated these tumors, flow sorted for GFPhigh and GFPlow tumor cells and subjected these populations to gene expression profiling.
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| Contributor(s) |
Horst D, Shivdasani RA |
| Citation(s) |
22318865 |
| |
| Submission date |
Sep 27, 2011 |
| Last update date |
Dec 06, 2018 |
| Contact name |
David Horst |
| E-mail(s) |
david.horst@med.uni-muenchen.de
|
| Organization name |
Pathologisches Institut der LMU
|
| Street address |
Thalkirchner Str. 36
|
| City |
Munich |
| ZIP/Postal code |
80337 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL571 |
[HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array |
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| Samples (10)
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| GSM802195 |
Caco2 GFPhigh cells, biological rep1 |
| GSM802196 |
Caco2 GFPhigh cells, biological rep2 |
| GSM802197 |
Caco2 GFPhigh cells, biological rep3 |
| GSM802198 |
Caco2 GFPlow cells, biological rep1 |
| GSM802199 |
Caco2 GFPlow cells, biological rep2 |
| GSM802200 |
Caco2 GFPlow cells, biological rep3 |
| GSM802201 |
primary colon cancer GFPhigh cells, biological rep1 |
| GSM802202 |
primary colon cancer GFPhigh cells, biological rep2 |
| GSM802203 |
primary colon cancer GFPlow cells, biological rep1 |
| GSM802204 |
primary colon cancer GFPlow cells, biological rep2 |
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| Relations |
| BioProject |
PRJNA146385 |
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