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Series GSE32408 Query DataSets for GSE32408
Status Public on Feb 16, 2012
Title Expression data from TOP-GFP sorted colon cancer cells
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Colon cancers typically contain tumor cell populations with differential WNT signaling activity. Colon cancer cells with high WNT-activity have been attributed increase tumorigenic potential and stem cell characteristics.
We extracted tumor cells with differential WNT activity using fluorescent reporters from xenografted human colon cancer cell line tumors and primary human colon cancer xenografts and analysed their gene expression profiles.
Overall design Colon cancer cell lines and primary colon cancer xenografts were transduced with lentiviral TOP-GFP reporters, and grown as xenografts in NOD-SCID mice. We disaggregated these tumors, flow sorted for GFPhigh and GFPlow tumor cells and subjected these populations to gene expression profiling.
Contributor(s) Horst D, Shivdasani RA
Citation(s) 22318865
Submission date Sep 27, 2011
Last update date Dec 06, 2018
Contact name David Horst
Organization name Pathologisches Institut der LMU
Street address Thalkirchner Str. 36
City Munich
ZIP/Postal code 80337
Country Germany
Platforms (1)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
Samples (10)
GSM802195 Caco2 GFPhigh cells, biological rep1
GSM802196 Caco2 GFPhigh cells, biological rep2
GSM802197 Caco2 GFPhigh cells, biological rep3
BioProject PRJNA146385

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Supplementary file Size Download File type/resource
GSE32408_RAW.tar 23.8 Mb (http)(custom) TAR (of CEL, CHP)
Processed data included within Sample table

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