|
| Status |
Public on Sep 26, 2011 |
| Title |
DNA methylation and gene expression changes during human embryonic stem cell differentiation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
Methylation profiling by genome tiling array
|
| Summary |
Investigate the genome-wide DNA methylation and gene expression changes during human embryonic stem cell differentiation.
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| |
|
| Overall design |
hESCs (H1 and H13) DNA samples for 3 different differentian stage (D0, undifferentiated;D21, 21 days after undirected differentiation; D90, 90 days after undirected differentiation); two-color experiment, paired comparison (differentiated vs undifferentiated; A & B: two biological replicates). Differentian-stage variation in methylation profiling of human DNA samples were detected by MSAM. 2µg of DNA was serially digested with SmaI and XmaI followed by an adaptor ligation and adaptor mediated PCR amplification. Analysis of differentian-stage variation in gene expression of human mRNA samples were implementd following standard Agilent protocol.
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| |
|
| Contributor(s) |
Shen L, Zhang W |
| Citation(s) |
23459939 |
| |
| Submission date |
Sep 26, 2011 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Robert A Waterland |
| E-mail(s) |
waterlan@bcm.tmc.edu
|
| Organization name |
Baylor College of Medicine
|
| Street address |
1100 Bates St.,
|
| City |
Houston |
| State/province |
TX |
| ZIP/Postal code |
77030 |
| Country |
USA |
| |
|
| Platforms (2) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
| GPL14617 |
Agilent-017777 Whole Human Genome (hg18) Custom 1×244k Array (Feature Number version) |
|
| Samples (16)
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|
| Relations |
| BioProject |
PRJNA147971 |
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