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| Status |
Public on Sep 15, 2014 |
| Title |
Up-regulated aldo-ketoreductase1B10 in chronic hepatitis C: Association with serum alpha-fetoprotein and hepatocellular carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
We attempted to identify a specific gene expression signature by performing microarray analysis on the livers of patients with chronic hepatitis C and AFP elevation. Distinct hepatic gene expression patterns were demonstrated between patients with elevated AFP (≥10ng/mL) and normal AFP (<10ng/mL). Among the 30,150 valid genes, 627 were identified as differentially expressed genes with a minimal fold change of 2.0. Using these 627 genes, PCA and HCA were used to successfully distinguish samples according to their AFP status.
Of the 627 differently expressed genes, AKR1B10 was most abundantly expressed in patients with elevated AFP. AKR1B10 expression was validated by Real-time RT-PCR and immunohistochemical study. Furthermore, a proportional correlation between AKR1B10 expression and serum AFP was demonstrated by regression analysis. A matched case-control study indicated that AKR1B10 up-regulation was an independent risk factor for HCC development.
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| Overall design |
Liver tissues samples from 48 chronic hepatitis C patients were stratified by their serum AFP levels and analyzed for gene expression profiles. AKR1B10 was up-regulated in patients with chronic hepatitis C in association with serum AFP, and was an independent risk factor for HCC development.
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| Contributor(s) |
Sato S, Genda T, Hirano K, Tuzura H, Narita Y, Kanemitsu Y, Kikuchi T, Iijima K, Wada R, Ichida T |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 19, 2011 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Shunsuke Sato |
| E-mail(s) |
syusato@juntendo.ac.jp
|
| Phone |
81-5-5948-3111
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| Fax |
81-5-5948-5088
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| Organization name |
Juntendo University Shizuoka Hospital
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| Department |
Department of Internal Medicine
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| Lab |
Division of Gastroenterology and Hepatology
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| Street address |
1129 Nagaoka
|
| City |
Izunokuni |
| State/province |
Shizuoka |
| ZIP/Postal code |
410-2295 |
| Country |
Japan |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (48)
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| Relations |
| BioProject |
PRJNA147211 |
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