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Series GSE32220 Query DataSets for GSE32220
Status Public on Sep 19, 2011
Title Cell-type independent MYC target genes reveal a primordial signature involved in biomass accumulation
Organism Homo sapiens
Experiment type Expression profiling by array
Genome binding/occupancy profiling by genome tiling array
Summary The functions of key oncogenic transcription factors independent of context have not been fully delineated despite our richer understanding of the genetic alterations in human cancers. The MYC oncogene, which produces the Myc transcription factor, is frequently altered in human cancer and is a major regulatory hub for many cancers. In this regard, we sought to unravel the primordial signature of Myc function by using high-throughput genomic approaches to identify the cell-type independent core Myc target gene signature. Using a model of human B lymphoma cells bearing inducible MYC, we identified a stringent set of direct Myc target genes via chromatin immunoprecipitation (ChIP), global nuclear run-on assay, and changes in mRNA levels. We also identified direct Myc targets in human embryonic stem cells (ESCs). We further document that a Myc core signature (MCS) set of target genes is shared in mouse and human ESCs as well as in four other human cancer cell types. Remarkably, the expression of the MCS correlates with MYC expression in a cell-type independent manner across 8,129 microarray samples, which include 312 cell and tissue types. Furthermore, the expression of the MCS is elevated in vivo in Em-Myc transgenic murine lymphoma cells as compared with premalignant or normal B lymphocytes. Expression of the MCS in human B cell lymphomas, acute leukemia, lung cancers or Ewing sarcomas has the highest correlation with MYC expression. Annotation of this gene signature reveals Myc's primordial function in RNA processing, ribosome biogenesis and biomass accumulation as its key roles in cancer and stem cells.

This SuperSeries is composed of the SubSeries listed below.
Overall design Refer to individual Series
Citation(s) 22039435
Submission date Sep 19, 2011
Last update date Feb 18, 2019
Contact name Hongkai Ji
Organization name Johns Hopkins Bloomberg School of Public Health
Department Department of Biostatistics
Street address 615 North Wolfe Street, Rm E3638
City Baltimore
State/province MD
ZIP/Postal code 21205
Country USA
Platforms (3)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
GPL5082 [Hs_PromPR] Affymetrix Human Promoter 1.0R Array
GPL5175 [HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [transcript (gene) version]
Samples (15)
GSM791773 P493 anti Myc (SC)
GSM791774 P493 anti Myc (Epi)
GSM791775 H9 anti Myc
This SuperSeries is composed of the following SubSeries:
GSE31964 MYC binding in human B cells (P493-6) and ESC (H9)
GSE31965 Gene expression comparison between undifferentiated H9 cells and trophoblasts
GSE32219 Gene expression from human B-lymphocytes (P493-6)
BioProject PRJNA147219

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32220_RAW.tar 541.2 Mb (http)(custom) TAR (of BAR, CEL, TXT)

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