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Series GSE32184 Query DataSets for GSE32184
Status Public on Nov 30, 2011
Title MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing [deep sequencing]
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Non-coding RNA profiling by high throughput sequencing
Summary Here we show that MIWI is a small RNA-guided ribonuclease (Slicer) that requires extensive complementarity for target cleavage in vitro. Disruption of its catalytic activity in mice by a single point mutation results in male infertility and displays increased accumulation of LINE1 transposon transcripts.
Overall design MIWI-associated piRNAs from different genotypes were sequenced. Total RNA from purified round spermatids were subjected to Ribozero purification and strand-specific RNAseq lib prepared. Global 5' RACE library was prepare from indicated genotypes.
Contributor(s) Reuter M, Berninger P, Chuma S, Shah H, Hosokawa M, Funaya C, Antony C, Sachidanandam R, Pillai RS
Citation(s) 22121019
Submission date Sep 16, 2011
Last update date May 15, 2019
Contact name Ramesh Pillai
Organization name University of Geneva
Department Department of Molecular Biology
Street address 30, Quai Ernest-Ansermet
City Gneveva
ZIP/Postal code CH-1211
Country Switzerland
Platforms (2)
GPL9250 Illumina Genome Analyzer II (Mus musculus)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (12)
GSM822758 Testes, P14 Miwi +/+ [MR09]
GSM822759 Testes, P20 Miwi +/+ [MR10]
GSM822760 Testes, Adult Miwi +/+ [MR11]
This SubSeries is part of SuperSeries:
GSE32183 MIWI catalysis is required for piRNA amplification-independent LINE1 transposon silencing
SRA SRP009270
BioProject PRJNA154861

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