|Public on Sep 20, 2012
|Elf5 inhibits epithelial mesenchymal transition in development and cancer metastasis through transcriptional repression of Snail2
|Expression profiling by array
|Elf5 (or ESE-2) is an ETS transcription factor that is abundantly expressed in the mammary epithelium, where it plays a critical role in dictating cell fate and lineage choices. These changes are in part mediated by alterations in the expression and activity of critical components of the Jak/Stat pathway. While the biological function of Elf5 in mammary gland development has been well characterized, its role in breast cancer remains to be elucidated. Here we show that loss of Elf5 leads to features associated with epithelial-mesenchymal transition (EMT) in the mouse mammary gland during pregnancy and lactation. These cellular changes in Elf5-null mammary epithelia are also reflected at the molecular level by the global enrichment of EMT-related gene signatures. ELF5 is expressed in higher level in weakly metastatic breast cancer cells that retained epithelial features compared to highly metastatic cells with mesenchymal features. ELF5 knockdown in T47D breast cancer cells resulted in EMT and increased migration. Conversely, ectopic expression of Elf5 revert mesenchymal-like MDA-MB-231 cells and its lung-tropic variant LM2 to an epithelial phenotype, with reduced migration, invasion and lung metastatic abilities. Finally, we showed that Elf5 binds directly to the promoter of the EMT transcriptional factor Snail2 (Slug) and repress its expression. Taken together, these data established a novel function for Elf5 in inhibiting EMT in normal mammary epithelium and in breast cancer through direct targeting of Snail2.
This SuperSeries is composed of the SubSeries listed below.
|Refer to individual Series.
|Sep 15, 2011
|Last update date
|Feb 29, 2024
|LTL227 Wathington Road
|Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
|Control (GFP) infected LM2 cell, replicate 1
|ELF5 infected LM2 cell, replicate 1
|Control (GFP) infected LM2 cell, replicate 2
|This SuperSeries is composed of the following SubSeries:
|LM2 cell: infected with lentivirus to stably express Elf5 vs GFP
|MDA-MB-231 cell: infected with lentivirus to stably express mut-Elf5 vs WT-Elf5