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Series GSE32150 Query DataSets for GSE32150
Status Public on Sep 20, 2012
Title Elf5 inhibits epithelial mesenchymal transition in development and cancer metastasis through transcriptional repression of Snail2
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Elf5 (or ESE-2) is an ETS transcription factor that is abundantly expressed in the mammary epithelium, where it plays a critical role in dictating cell fate and lineage choices. These changes are in part mediated by alterations in the expression and activity of critical components of the Jak/Stat pathway. While the biological function of Elf5 in mammary gland development has been well characterized, its role in breast cancer remains to be elucidated. Here we show that loss of Elf5 leads to features associated with epithelial-mesenchymal transition (EMT) in the mouse mammary gland during pregnancy and lactation. These cellular changes in Elf5-null mammary epithelia are also reflected at the molecular level by the global enrichment of EMT-related gene signatures. ELF5 is expressed in higher level in weakly metastatic breast cancer cells that retained epithelial features compared to highly metastatic cells with mesenchymal features. ELF5 knockdown in T47D breast cancer cells resulted in EMT and increased migration. Conversely, ectopic expression of Elf5 revert mesenchymal-like MDA-MB-231 cells and its lung-tropic variant LM2 to an epithelial phenotype, with reduced migration, invasion and lung metastatic abilities. Finally, we showed that Elf5 binds directly to the promoter of the EMT transcriptional factor Snail2 (Slug) and repress its expression. Taken together, these data established a novel function for Elf5 in inhibiting EMT in normal mammary epithelium and in breast cancer through direct targeting of Snail2.

This SuperSeries is composed of the SubSeries listed below.
Overall design Refer to individual Series.
Citation(s) 23086238
Submission date Sep 15, 2011
Last update date Feb 29, 2024
Contact name Yong Wei
Organization name Princeton University
Department Molecular Biology
Lab Kang
Street address LTL227 Wathington Road
City Plainsboro
State/province NJ
ZIP/Postal code 08536
Country USA
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (10)
GSM796560 Control (GFP) infected LM2 cell, replicate 1
GSM796561 ELF5 infected LM2 cell, replicate 1
GSM796562 Control (GFP) infected LM2 cell, replicate 2
This SuperSeries is composed of the following SubSeries:
GSE32143 LM2 cell: infected with lentivirus to stably express Elf5 vs GFP
GSE32144 MDA-MB-231 cell: infected with lentivirus to stably express mut-Elf5 vs WT-Elf5
BioProject PRJNA147651

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE32150_RAW.tar 161.3 Mb (http)(custom) TAR (of TXT)

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