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| Status |
Public on Jul 11, 2012 |
| Title |
miRNA measurements in wildtype and Eri1-deficient Th cells |
| Platform organisms |
Caenorhabditis elegans; Drosophila melanogaster; Danio rerio; Gallus gallus; Homo sapiens; Mus musculus; Rattus norvegicus |
| Sample organism |
Mus musculus |
| Experiment type |
Non-coding RNA profiling by array
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| Summary |
Natural killer (NK) cells play a critical role in early host defense to infected and transformed cells. Here we show that mice deficient in Eri1, a conserved 3’-to-5’ exoribonuclease that represses RNA interference, have a cell-intrinsic defect in the homeostasis and terminal maturation of NK cells. Eri1–/– NK cells show delayed acquisition of Ly49 receptors in the bone marrow and a selective reduction in Ly49D and Ly49H activating receptors in the periphery. Furthermore, antigen-specific Ly49H+ NK cells deficient in Eri1 fail to expand efficiently in mouse cytomegalovirus (MCMV) infection. Consequently, we find that Eri1 is required for immune-mediated control of MCMV titers. In contrast to NK cells, T cells deficient in Eri1 differentiate into effectors that produce high levels of Th2 cytokines. Here we identify miRNAs as the major endogenous small RNA target of Eri1 in mouse lymphocytes. NK and T cells deficient in Eri1 display an approximately two-fold increase in all miRNAs. In Eri1-deficient T cells, ectopic expression of wildtype Eri1 is sufficient to rescue defects in both miRNA homeostasis and Th2 cytokine production. Thus mouse Eri1 negatively regulates miRNA homeostasis and is required to promote normal lymphocyte effector functions.
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| Overall design |
One sample; one color design. These are six arrays from a study investigating the microRNA profile of wildtype and Eri1-deficient Th cells that is to published separately.
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| Contributor(s) |
Thomas M, Ansel KM |
| Citation(s) |
22613798 |
| |
| Submission date |
Sep 14, 2011 |
| Last update date |
Jul 11, 2012 |
| Contact name |
Andrea J Barczak |
| Organization name |
UCSF Sandler Center Functional Genomics Core Facility
|
| Street address |
1550 4th St Rock Hall 545
|
| City |
San Francisco |
| State/province |
CA |
| ZIP/Postal code |
94143 |
| Country |
USA |
| |
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| Platforms (2) |
| GPL14557 |
Agilent-020736 miRNA-v3.1-7species-8x15K array |
| GPL14558 |
Agilent-021542 miRNA-v3.2-8x15K array |
|
| Samples (6)
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| GSM796410 |
Eri1 fl/fl CD4-cre conditional knockout Th2 Rep2 |
| GSM796411 |
Eri1 +/+ CD4-cre wildtype Th2 Rep3 |
| GSM796412 |
Eri1 fl/fl CD4-cre conditional knockout Th2 Rep3 |
|
| Relations |
| BioProject |
PRJNA147431 |
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