|
| Status |
Public on Nov 01, 2011 |
| Title |
Gene expression profiling of human vascular cells following gene transfer |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
For further understanding of the gene expression profile of human vascular cells following gene transfer in vitro, we have employed whole genome microarray expression profiling as a discovery platform to identify gene families or single genes with the potential to distinguish between responders and non-responders to a particular gene therapy. Human vascular cells from deseased donors were derived and transduced in vitro. Total RNA was isolated from the cells and subjected to microarray analysis using the Agilent technology.
|
| |
|
| Overall design |
Two human donors, with two cell types per donor, were tested, including transduced cells and two different control samples. Technical triplicates were tested for each sample type. Thus, a total of 36 whole genome arrays were processed and analyzed. Expression of candidate genes from this signature will be quantified in the same RNA samples by real-time PCR, allowing further simplified analysis of a large pool of human donors.
|
| |
|
| Contributor(s) |
Gershtein D |
| Citation(s) |
26584888 |
| |
| Submission date |
Sep 08, 2011 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Diana Gershtein |
| Organization name |
MGVS
|
| Street address |
Bar Yehuda Rd
|
| City |
Nesher |
| ZIP/Postal code |
36660 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
|
| Samples (36)
|
| GSM792055 |
Donor 1 endothelial cells Ang rep 1 |
| GSM792056 |
Donor 1 endothelial cells Ang rep 2 |
| GSM792057 |
Donor 1 endothelial cells Ang rep 3 |
|
| Relations |
| BioProject |
PRJNA144963 |
Less...
More...