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Status |
Public on Dec 04, 2012 |
Title |
Creation of an anti-inflammatory GR cistrome by TLR4 signaling |
Organism |
Mus musculus |
Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
Summary |
An unresolved molecular paradox is how the glucocorticoid receptor (GR) activates some genes while potently repressing others. We carried out genome-wide localization and expression profiling experiments in primary bone marrow-derived mouse macrophages treated with Dexamethasone in the presence or absence of LPS. Unexpectedly, we find that the anti-inflammatory GR cistrome, which is principally composed of 'canonical' GREs colocalizing with NFkB and AP-1 co-enriched with the myeloid lineage factors C/EBP and Pu.1, is shaped by TLR4-directed chromatin dynamics, suggesting that context rather than sequence may be a critical determinant of function.
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Overall design |
Identification of GR, cJun, NFkB(p65) binding sites in primary bone-marrow derived macrophages unstimulated and LPS-stimulated (3hrs) that were untreated or pre-treated with Dexamethasone for 16 hrs
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Contributor(s) |
Yu RT, Uhlenhaut NH, Evans RM |
Citation(s) |
23159735 |
Submission date |
Aug 31, 2011 |
Last update date |
May 15, 2019 |
Contact name |
Ruth T Yu |
Organization name |
Salk Institute
|
Department |
Gene Expression Lab
|
Lab |
Ronald Evans
|
Street address |
10010 N Torrey Pines Rd
|
City |
La Jolla |
State/province |
CA |
ZIP/Postal code |
92037 |
Country |
USA |
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Platforms (1) |
GPL9250 |
Illumina Genome Analyzer II (Mus musculus) |
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Samples (9)
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Relations |
SRA |
SRP008124 |
BioProject |
PRJNA145469 |