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Series GSE31794 Query DataSets for GSE31794
Status Public on Dec 16, 2011
Title Factors influencing in vivo deposition of histone variants H3.1 and H3.3
Organism Homo sapiens
Experiment type Genome binding/occupancy profiling by high throughput sequencing
Summary Establishment of a proper chromatin landscape is central to genome function. Here, we explain H3 variant distribution by specific targeting and dynamics of deposition involving the CAF-1 and HIRA histone chaperones. Impairing replicative H3.1 incorporation via CAF-1 enables an alternative H3.3 deposition at replication sites via HIRA. Conversely, the H3.3 incorporation throughout the cell cycle via HIRA cannot be replaced by H3.1. ChIP-seq analyses reveal correlation between HIRA-dependent H3.3 accumulation and RNA pol II at transcription sites and specific regulatory elements, further supported by their biochemical association. Remarkably, the HIRA complex shows unique DNA binding properties and depleting HIRA increases DNA sensitivity to nucleases. We propose that protective gap-filling of naked DNA by HIRA leads to a broad distribution of H3.3, and HIRA association with Pol II ensures local H3.3 enrichment at specific sites.
Overall design Examination of genome-wide localization of two histone H3 variants.
Contributor(s) Almouzni G, Woolfe AD
Citation(s) 22195966
Submission date Aug 31, 2011
Last update date May 15, 2019
Contact name Genevieve Almouzni
Phone +33156246701
Organization name Institut Curie
Department Nuclear Dynamics and Genome Plasticity
Lab Chromatin dynamics
Street address 26 Rue D'Ulm
City Paris
State/province Ille De France
ZIP/Postal code 75005
Country France
Platforms (1)
GPL10999 Illumina Genome Analyzer IIx (Homo sapiens)
Samples (4)
GSM788633 H3.3 IP
GSM788634 H3.1 IP
GSM788635 input H3.3
SRA SRP008014
BioProject PRJNA145265

Download family Format
SOFT formatted family file(s) SOFTHelp
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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE31794_RAW.tar 1.5 Gb (http)(custom) TAR (of BED, WIG)
SRA Run SelectorHelp
Raw data are available in SRA
Processed data provided as supplementary file

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