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Status |
Public on Mar 01, 2012 |
Title |
Transcriptome Response of Leukocytes from Chickens Infected with Avian Pathogenic Escherichia coli Identifies Pathways Associated with Resistance |
Organism |
Gallus gallus |
Experiment type |
Expression profiling by array
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Summary |
Gene expression profiling of peripheral blood leukocytes in male broiler chickens exposed to APEC O1. Comparisons were made between Day 1 and Day 5 of all treatment groups, between differences in pathology and effect of vaccine on spleen gene expression. The goal was to determine expression differences that could convey genetic resistance to APEC O1.
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Overall design |
Chickens were either challenged or non-challenged with APEC, vaccinated or non-vaccinated, with blood collected 1 or 5 days post challenge. The non-vaccinated, challenged group was further subdivided into mild and severe pathologay based on internal lesion scores. This created 10 groups, done in 4 replicates. The non-vaccinated, non-challenged, day 1 group was used as the reference for all other samples. Peripheral blood leukocytes were isolated from whole blood for analysis.
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Contributor(s) |
Sandford EE, Orr M, Shelby M, Li X, Zhou H, Johnson TJ, Kariyawasam S, Liu P, Nolan LK, Lamont SJ |
Citation missing |
Has this study been published? Please login to update or notify GEO. |
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Submission date |
Aug 15, 2011 |
Last update date |
Mar 23, 2012 |
Contact name |
Susan J Lamont |
E-mail(s) |
sjlamont@iastate.edu
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Organization name |
Iowa State University
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Department |
Animal Science
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Street address |
2255 Kildee Hall
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City |
Ames |
State/province |
IA |
ZIP/Postal code |
50011 |
Country |
USA |
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Platforms (1) |
GPL6413 |
Chicken 44K custom Agilent microarray (2nd version) |
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Samples (36)
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GSM778627 |
Non-vaccinated, non-challenged, day 5, replicate 1 |
GSM778628 |
Non-vaccinated, challenged, day 1, severe, replicate 1 |
GSM778629 |
Vaccinated, challenged, day 1, replicate 1 |
GSM778630 |
Vaccinated, challenged, day 5, replicate 1 |
GSM778631 |
Non-vaccinated, challenged, day 5, mild, replicate 1 |
GSM778632 |
Non-vaccinated, challenged, day 1, mild, replicate 1 |
GSM778633 |
Vaccinated, non-challenged, day 5, replicate 1 |
GSM778634 |
Non-vaccinated, challenged, day 5, severe, replicate 1 |
GSM778635 |
Vaccinated, non-challenged, day 1, replicate 1 |
GSM778636 |
Vaccinated, non-challenged, day 5, replicate 2 |
GSM778637 |
Non-vaccinated, challenged, day 5, severe, replicate 2 |
GSM778638 |
Non-vaccinated, challenged, day 1, mild, replicate 2 |
GSM778639 |
Vaccinated, challenged, day 1, replicate 2 |
GSM778640 |
Vaccinated, challenged, day 5, replicate 2 |
GSM778641 |
Non-vaccinated, challenged, day 5, mild, replicate 2 |
GSM778642 |
Vaccinated, non-challenged, day 1, replicate 2 |
GSM778643 |
Non-vaccinated, challenged, day 1, severe, replicate 2 |
GSM778644 |
Non-vaccinated, non-challenged, day 5, replicate 2 |
GSM778645 |
Non-vaccinated, challenged, day 1, mild, replicate 3 |
GSM778646 |
Vaccinated, challenged, day 1, replicate 3 |
GSM778647 |
Vaccinated, non-challenged, day 5, replicate 3 |
GSM778648 |
Non-vaccinated, challenged, day 5, mild, replicate 3 |
GSM778649 |
Vaccinated, non-challenged, day 1, replicate 3 |
GSM778650 |
Non-vaccinated, non-challenged, day 5, replicate 3 |
GSM778651 |
Vaccinated, challenged, day 5, replicate 3 |
GSM778652 |
Non-vaccinated, challenged, day 5, severe, replicate 3 |
GSM778653 |
Non-vaccinated, challenged, day 1, severe, replicate 3 |
GSM778654 |
Vaccinated, non-challenged, day 5, replicate 4 |
GSM778655 |
Non-vaccinated, challenged, day 5, severe, replicate 4 |
GSM778656 |
Vaccinated, challenged, day 1, replicate 4 |
GSM778657 |
Non-vaccinated, non-challenged, day 5, replicate 4 |
GSM778658 |
Non-vaccinated, challenged, day 1, severe, replicate 4 |
GSM778659 |
Non-vaccinated, challenged, day 1, mild, replicate 4 |
GSM778660 |
Vaccinated, non-challenged, day 1, replicate 4 |
GSM778661 |
Vaccinated, challenged, day 5, replicate 4 |
GSM778662 |
Non-vaccinated, challenged, day 5, mild, replicate 4 |
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Relations |
BioProject |
PRJNA145915 |
Supplementary file |
Size |
Download |
File type/resource |
GSE31387_RAW.tar |
134.7 Mb |
(http)(custom) |
TAR (of TXT) |
Processed data included within Sample table |
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