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Series GSE3113 Query DataSets for GSE3113
Status Public on Aug 13, 2005
Title KLF4 Time Course
Organism Homo sapiens
Experiment type Expression profiling by array
Summary A stably transfected inducible system for KLF4 was established in RKO cells as previously described (Chen et al., 2001; Chen et al., 2003). This cell line, called EcR-RKO/KLF4, contains stably transfected receptors for the insect hormone, ecdysone (EcR), and a full-length mouse KLF4 cDNA driven by a promoter that responds to ecdysone and its analogue, ponasterone A. Cells were treated with 5 µM ponasterone A (PA), for various time durations. To control for the experiment, the vehicle, ethanol, was added for the same periods of time.
Keywords: Time course
Overall design RNA was extracted from EcR-RKO/KLF4 cells treated with ponasterone A or vehicle control for 0, 1, 2, 4, 6, 8, 12, and 24 hr, using Trizol (Invitrogen). RNA from each time point labeled with biotin using the Enzo BioArrayTM HighYieldTM RNA Transcript Labeling kit (T7), and hybridized to Affymetrix commercial HU133A chips. Hybridization was done using the GeneChip® Fluidics Station according the Affymetrix single stain protocol. The data were scanned and analyzed by an Affymetrix GeneChip® Fluidics Station 400 scanner and Affymetrix Microarray Suite 2.
Contributor(s) Yang VW, Whitney EM, Chen X
Citation(s) 17017123
Submission date Aug 11, 2005
Last update date Aug 10, 2018
Contact name Vincent W. Yang
Phone (404) 727-5638
Organization name Emory University
Department Digestive Diseases Division
Street address 201 Whitehead Research Building 615 Michael Street
City Atlanta
State/province GA
ZIP/Postal code 30322
Country USA
Platforms (1)
GPL96 [HG-U133A] Affymetrix Human Genome U133A Array
Samples (15)
GSM69661 0 hr KLF4 Time Course
GSM69662 1 hr KLF4 Time Course Induced
GSM69663 2 hr KLF4 Time Course Induced
BioProject PRJNA93121

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