|
| Status |
Public on Aug 15, 2011 |
| Title |
Context-Specific Regulation of NF-κB Target Gene Expression by EZH2 in Breast Cancers |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Both EZH2 and NF-κB contribute to aggressive breast cancer, yet whether the two oncogenic factors have functional cross-talk in breast cancer is largely unknown. Here, we uncover an unexpected role of EZH2 in conferring the constitutive activation of NF-κB target gene expression in ER-negative basal-like breast cancer cells. This function of EZH2 is independent of its histone methyltransferase activity but requires the physical interaction with RelA/RelB to promote the expression of NF-κB targets. Intriguingly, EZH2 acts oppositely in repressing NF-κB targets in ER-positive luminal-like breast cancer cells by interacting with ER and directing repressive histone methylation. Thus, EZH2 function as a double-facet molecule in breast cancers, functioning either as a transcriptional activator or repressor of NF-κB targets, in a cell context-dependent manner. These findings reveals an additional mechanism by which EZH2 promotes breast cancer progression and also underscore the need for developing context-specific strategy for therapeutic targeting of EZH2 in breast cancers.
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| |
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| Overall design |
12 samples were analyzed including three replicates of siNC CTRL and siEZH2 CTRL.
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| Contributor(s) |
Lee ST, Li Z, Wu Z, Aau M, Guan P, Karuturi RK, Liou YC, Yu Q |
| Citation(s) |
21884980 |
| |
| Submission date |
Jul 14, 2011 |
| Last update date |
Jan 18, 2013 |
| Contact name |
Peiyong Guan |
| E-mail(s) |
p.g@u.duke.nus.edu
|
| Organization name |
Genome Institute of Singapore
|
| Street address |
60 Biopolis Street, Genome, #02-01
|
| City |
Singapore |
| ZIP/Postal code |
138672 |
| Country |
Singapore |
| |
|
| Platforms (1) |
| GPL6104 |
Illumina humanRef-8 v2.0 expression beadchip |
|
| Samples (12)
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| Relations |
| BioProject |
PRJNA144449 |
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