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Series GSE30549 Query DataSets for GSE30549
Status Public on Sep 23, 2011
Title An Extended dsRBD with a Novel Zinc-Binding Motif Mediates Nuclear Retention of Fission Yeast Dicer
Organism Schizosaccharomyces pombe
Experiment type Non-coding RNA profiling by high throughput sequencing
Summary Dicer proteins function in RNA interference (RNAi) pathways by generating small RNAs (sRNAs). Here we report the solution structure of the C-terminal domain of Schizosaccharomyces pombe Dicer (Dcr1). The structure reveals an unusual double-stranded RNA binding domain (dsRBD) fold embedding a novel zinc-binding motif that is conserved among dicers in yeast. Although the C-terminal domain of Dcr1 still binds nucleic acids, this property is dispensable for proper functioning of Dcr1. In contrast, disruption of zinc coordination renders Dcr1 mainly cytoplasmic and leads to remarkable changes in gene expression and loss of heterochromatin assembly. In summary, our results reveal novel insights into the mechanism of nuclear retention of Dcr1 and raise the possibility that this new class of dsRBDs might generally function in nucleo-cytoplasmic trafficking and not substrate binding. The C-terminal domain of Dcr1 constitutes a novel regulatory module that might represent a potential target for therapeutic intervention with fungal diseases.
Overall design Small RNA libraries from total RNA isolations of wild-type, dcr1Delta, dcr1SHSS, and dcr1Deltaloop2 cells and subjected to high-throughput sequencing.
Contributor(s) Barraud P, Emmerth S, Shimada Y, Hotz H, Allain FH, Buhler M
Citation(s) 21847092
Submission date Jul 11, 2011
Last update date May 15, 2019
Contact name Dimos Gaidatzis
Organization name Friedrich Miescher Institute
Street address Maulbeerstrasse 66
City Basel
ZIP/Postal code 4058
Country Switzerland
Platforms (1)
GPL9453 Illumina Genome Analyzer II (Schizosaccharomyces pombe)
Samples (4)
GSM757894 wild-type
GSM757895 dcr1Deltaloop2
GSM757896 dcr1-SHSS
SRA SRP007460
BioProject PRJNA144481

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