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Series GSE30547 Query DataSets for GSE30547
Status Public on Dec 01, 2011
Title Expression data from the Arabidopsis root epidermis
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary The root epidermis of Arabidopsis provides a simple and experimentally useful model for studying the molecular basis of cell fate and differentiation. The goal of this study was to define the larger gene regulatory network that governs the differentiation of the root hair and non-hair cell types of the Arabidopsis root epidermis. Transcript levels in the root epidermis of wild-type and mutant lines were assessed by purifying populations of root epidermal cells using fluorescence-based cell-sorting. Further, the role of the plant hormones auxin and ethylene on root epidermis development was assessed by defining transcript levels in the root epidermis of plants grown on media containing IAA or ACC. These microarray results were used to construct a comprehensive gene regulatory network that depicts the transcriptional control of root epidermal cell fate and differentiation in Arabidopsis.
 
Overall design The cells of the developing root epidermis were obtained by growing plant seedlings expressing the WER::GFP transgene under sterile conditions on MS media, cutting off root tips (including all developmental stages), protoplasting the roots, and purifying cells containing GFP using FACS. The WER::GFP transgene is expressed throughout the developing cells of the root epidermis and the lateral root cap. Three biological replicates were analyzed for each of the 25 plant lines examined in this study.
 
Contributor(s) Schiefelbein J, Bruex A, Kainkaryam R, Woolf P
Citation(s) 22253603
Submission date Jul 11, 2011
Last update date Sep 20, 2012
Contact name John Schiefelbein
E-mail(s) schiefel@umich.edu
Phone 734-764-3580
Organization name University of Michigan
Department Molecular, Cellular, and Developmental Biology
Street address 830 North University Avenue
City Ann Arbor
State/province Michigan
ZIP/Postal code 48109
Country USA
 
Platforms (1)
GPL13838 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array [CDF: Brainarray TAIRG, Version 12.1.0]
Samples (75)
GSM757819 cpc try mutant, biological rep1 at Duke
GSM757820 cpc try mutant, biological rep2 at Duke
GSM757821 cpc try mutant, biological rep3 at Duke
Relations
BioProject PRJNA144479

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Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30547_RAW.tar 173.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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