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Series GSE30223 Query DataSets for GSE30223
Status Public on Sep 09, 2011
Title Expression data of germinating Arabidopsis seeds
Organism Arabidopsis thaliana
Experiment type Expression profiling by array
Summary In depth temporal profiling of transcript changes at 10 time points during germination in Arabidopsis seed was carried out. The time course utilised, encompassed seed maturation, stratification, germination and post-germination and provided a global investigation into the tightly regulated, phasic changes that define seed germination.
A previously unidentified transient expression pattern was identified for a group of genes, whereby a significant rise in abundance was observed at the end of stratification and significantly lower expression observed up to 6 hours later.
 
Overall design Total RNA extraction was carried out on 80 mg of Arabidopsis seeds at 10 time points during germination in triplicate. The time points selected were: freshly harvested seed (H), seeds following 15 days of ripening (0 h), seeds after; 1 h of stratification (1 h S), 12 h of stratification (12 h S), 48 h of stratification (48 h S), followed by seed collected 1 hour into the light (1 h SL), 6 hours into the light (6 h SL), 12 hours into the light (12 h SL), 24 hours into the light (24 h SL) and 48 hours into the light (48 h SL).
 
Contributor(s) Narsai R, Law SR, Carrie C, Xiu L, Whelan J
Citation(s) 21908688, 24101922
Submission date Jun 27, 2011
Last update date Feb 05, 2020
Contact name Simon R. Law
E-mail(s) simon.law@umu.se
Organization name Umeå Plant Science Centre
Lab Keech
Street address Umeå University
City Umeå
State/province Västerbotten
ZIP/Postal code 90734
Country Sweden
 
Platforms (1)
GPL198 [ATH1-121501] Affymetrix Arabidopsis ATH1 Genome Array
Samples (30)
GSM748466 seed freshly harvested H Replicate 1
GSM748467 seed freshly harvested H Replicate 2
GSM748468 seed freshly harvested H Replicate 3
Relations
Affiliated with GSE69995
BioProject PRJNA143719

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Supplementary file Size Download File type/resource
GSE30223_RAW.tar 58.2 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table
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