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Series GSE30182 Query DataSets for GSE30182
Status Public on Nov 02, 2012
Title COLO320 DNA copy number analysis
Organism Homo sapiens
Experiment type Genome variation profiling by SNP array
Summary Somatic DNA alteration underlies tumor development and progression, and gives rise to tumors with diverse genetic contexts. Here, we identify in a collection of 29 colorectal cancer cell lines and 226 primary colorectal tumors recurrent amplification of chromosome 13, an alteration highly restricted to colorectal-derived cancers. A minimal region of amplification on 13q12.2 pinpoints caudal type homeobox transcription factor CDX2, a master regulator of anterior-posterior patterning, midgut development, and intestinal epithelial cell differentiation and maintenance. In contrast to its described role as a colorectal tumor suppressor, we show that in the context of genomic amplification, CDX2 is required for proliferation and anchorage-independent growth of colorectal cancer cells. By genome-wide expression and location analysis, we reveal that CDX2 directly promotes expression of Wnt pathway genes. Further results suggest that CDX2 induces expression of intestinal differentiation markers and modulates b-catenin transcriptional activity. These data characterize CDX2 as a novel lineage-survival oncogene deregulated in colorectal cancer.
Overall design Genome-wide DNA copy number analysis of cell line COLO320 by Affymetrix SNP 6.0 array
Contributor(s) Salari K, Pollack JR
Citation(s) 23112155
Submission date Jun 23, 2011
Last update date Nov 27, 2018
Contact name Keyan Salari
Organization name Massachusetts General Hospital
Street address Gray/Bigelow Rm 1102, 55 Fruit Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
Platforms (1)
GPL6801 [GenomeWideSNP_6] Affymetrix Genome-Wide Human SNP 6.0 Array
Samples (1)
GSM747237 COLO320 DNA copy number
This SubSeries is part of SuperSeries:
GSE30475 Colorectal Cancer Cells
BioProject PRJNA155107

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30182_RAW.tar 61.7 Mb (http)(custom) TAR (of CEL, CNCHP)
Processed data included within Sample table
Processed data are available on Series record

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