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Series GSE30181 Query DataSets for GSE30181
Status Public on Nov 02, 2012
Title COLO320 cells: siCDX2 vs. non-targeting control
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Somatic DNA alteration underlies tumor development and progression, and gives rise to tumors with diverse genetic contexts. Here, we identify in a collection of 29 colorectal cancer cell lines and 226 primary colorectal tumors recurrent amplification of chromosome 13, an alteration highly restricted to colorectal-derived cancers. A minimal region of amplification on 13q12.2 pinpoints caudal type homeobox transcription factor CDX2, a master regulator of anterior-posterior patterning, midgut development, and intestinal epithelial cell differentiation and maintenance. In contrast to its described role as a colorectal tumor suppressor, we show that in the context of genomic amplification, CDX2 is required for proliferation and anchorage-independent growth of colorectal cancer cells. By genome-wide expression and location analysis, we reveal that CDX2 directly promotes expression of Wnt pathway genes. Further results suggest that CDX2 induces expression of intestinal differentiation markers and modulates b-catenin transcriptional activity. These data characterize CDX2 as a novel lineage-survival oncogene deregulated in colorectal cancer.
Overall design Two-condition experiment, CDX2 siRNA knockdown vs. control, using two independent siRNAs against CDX2
Contributor(s) Salari K, Pollack JR
Citation(s) 23112155
Submission date Jun 23, 2011
Last update date Feb 22, 2018
Contact name Keyan Salari
Organization name Massachusetts General Hospital
Street address Gray/Bigelow Rm 1102, 55 Fruit Street
City Boston
State/province MA
ZIP/Postal code 02114
Country USA
Platforms (1)
GPL4133 Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version)
Samples (2)
GSM747235 COLO320 CDX2 siRNA #6 24hr
GSM747236 COLO320 CDX2 siRNA #8 24hr
This SubSeries is part of SuperSeries:
GSE30475 Colorectal Cancer Cells
BioProject PRJNA155087

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE30181_RAW.tar 15.4 Mb (http)(custom) TAR (of TXT)
Processed data included within Sample table

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