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| Status |
Public on Mar 14, 2014 |
| Title |
Expression profiles of X-ray responsive miRNA in human cells [mRNA data] |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
MicroRNA (miRNA) is a type of non-coding RNA that regulates the expression of its target genes by interacting with the complementary sequence of the target mRNA molecules. Recent evidence has shown that genotoxic stress induces miRNA expression, but the target genes involved and role in cellular responses remain unclear. We examined the role of miRNA in the cellular response to X-ray irradiation by studying the expression profiles of radio-responsive miRNAs and their target genes in cultured human cell lines. We found that expression of miR-574-3p was induced in the lung cancer cell line A549 by X-ray irradiation. Overexpression of miR-574-3p caused delayed growth in A549 cells. A predicted target site was detected in the 3'-untranslated region of the enhancer of the rudimentary homolog (ERH) gene, and transfected cells showed an interaction between the luciferase reporter containing the target sequences and miR-574-3p. Overexpression of miR-574-3p suppressed ERH protein production and delayed cell growth. This delay was confirmed by knockdown of ERH expression. Our study suggests that miR-574-3p may contribute to the regulation of the cell cycle in response to X-ray irradiation via suppression of ERH protein production.
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| Overall design |
mRNA expression were compared between the A549 cell overexpressing a radio-responsive miRNA and in the A549 cells suppressing the miRNA. Five miRNAs (miR-181d, miR-565, miR-574-3p, miR-629* and miR-766) were examined. Microarray experiments were performed with triplicate for each experiment.
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| Contributor(s) |
Ishikawa K, Ishikawa A, Imai T |
| Citation(s) |
24566139 |
| |
| Submission date |
Jun 21, 2011 |
| Last update date |
Feb 22, 2018 |
| Contact name |
Ken-ichi Ishikawa |
| E-mail(s) |
k_ishi@nirs.go.jp
|
| Phone |
+81-43-206-3072
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| Organization name |
National Institute of Radiological Sciences
|
| Department |
Research Center for Charged Particle Therapy
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| Lab |
Advanced Radiation Biology Research Program
|
| Street address |
4-9-1, Anagawa, Inage-ku
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| City |
Chiba |
| State/province |
Chiba |
| ZIP/Postal code |
263-8555 |
| Country |
Japan |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (15)
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| This SubSeries is part of SuperSeries: |
| GSE30132 |
Expression profiles of X-ray responsive miRNA in human cells |
|
| Relations |
| BioProject |
PRJNA155199 |
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