 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Jun 14, 2011 |
| Title |
Continued studies the glycosylation changes in a cell line model of embryo implantation of steroid-exposed and control Ichikawa cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
This experiment continues our investigation of glycosylation in endometrial epithelium. During the menstrual cycle, estrogen and progesterone sensitise the uterus to the implanting embryo. There is a major expansion of Golgi and secretory function with increases in mucin (MUC1) and the appearance of specific glycans. We are using Ishikawa cells as a model for in vitro implantation, and have shown the presence of steroid receptors and alterations in glycosylation after hormone exposure.
|
| |
|
| Overall design |
We are using Ishikawa cells as a model for in vitro implantation, and have shown the presence of steroid receptors and alterations in glycosylation after hormone exposure. We now wish to profile glycogenes in steroid (E+P) exposed and control cells.
We now wish to profile glycogenes in steroid (E+P) exposed and control cells. Steroid conditions are: estradiol (E2 at 10exp -8M) and medroxyprogesterone acetate (MPA at 10 exp -7M)): 24h with E2, followed by E2+MPA for 72h. Cells were grown on plastic or glass.
|
| |
|
| Contributor(s) |
Aplin J, Head SR |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Jun 13, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Steven Head |
| E-mail(s) |
shead@scripps.edu
|
| Phone |
858 784-2263
|
| Organization name |
The Scripps Research Institute
|
| Street address |
10550 N Torrey Pines Rd
|
| City |
La Jolla |
| State/province |
CA |
| ZIP/Postal code |
92037 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL11095 |
[GLYCOv3_Hs] Custom Affymetrix Glyco v3 GeneChip |
|
| Samples (12)
|
| GSM741118 |
1CON1: Control, condition 1, triplicate #1 |
| GSM741119 |
2CON1: Control, condition 1, triplicate #2 |
| GSM741120 |
31CON1: Control, condition 1, triplicate #3 |
| GSM741121 |
4EXP1: Experiment 1, condition 1, triplicate #1 |
| GSM741122 |
5EXP1: Experiment 1, condition 1, triplicate #2 |
| GSM741123 |
6EXP1: Experiment 1, condition 1, triplicate #3 |
| GSM741124 |
7CON2: Control, condition 2, triplicate #1 |
| GSM741125 |
8CON2: Control, condition 2, triplicate #2 |
| GSM741126 |
9CON2: Control, condition 2, triplicate #3 |
| GSM741127 |
10EXP2: Experiment 2, condition 2, triplicate #1 |
| GSM741128 |
11EXP2: Experiment 2, condition 2, triplicate #2 |
| GSM741129 |
12EXP2: Experiment 2, condition 2, triplicate #3 |
|
| Relations |
| BioProject |
PRJNA140773 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE29940_RAW.tar |
9.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...