|
| Status |
Public on Jul 22, 2005 |
| Title |
Entamoeba plus Caco2 cells |
| Organism |
Entamoeba histolytica |
| Experiment type |
Expression profiling by array
|
| Summary |
Entamoeba histolytica is a protozoan parasite which causes colitis and liver abscesses. Using a genomic DNA microarray consisting of 1.6 - 2.0 kb genomic inserts we have generated a transcriptional profile of 1,971 unique parasite transcripts. The arrays in this experiment set were used to (1) estimate relative transcript abundance for Entamoeba histolytica (HM-1:IMSS) trophozoites in mid-logarithmic growth and (2) to examine changes in the transcriptional profile of Entamoeba histolytica when the parasite interacts with colonic epithelial cells (Caco-2). A time course was used such that RNA was isolated from ameba alone and ameba + Caco-2 cells at 3hrs, 6hrs and 9hrs corresponding to 10%, 50% and 90% cell monolayer destruction. At least two biological experiments and three replicates were used for each time point.
Groups of assays that are related as part of a time series.
Keywords: time_series_design
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| |
|
| Overall design |
User Defined
|
| |
|
| Contributor(s) |
Macfarlane R |
| Citation(s) |
15826645 |
| |
| Submission date |
Jul 21, 2005 |
| Last update date |
Mar 17, 2012 |
| Organization |
Stanford Microarray Database (SMD) |
| E-mail(s) |
array@genome.stanford.edu
|
| Phone |
650-498-6012
|
| URL |
http://genome-www5.stanford.edu/
|
| Department |
Stanford University, School of Medicine
|
| Street address |
300 Pasteur Drive
|
| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
| |
|
| Platforms (1) |
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| Samples (21)
|
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| This SubSeries is part of SuperSeries: |
| GSE4400 |
Transcriptional profiling of Entamoeba histolytica trophozoites |
|
| Relations |
| BioProject |
PRJNA104775 |
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