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| Status |
Public on Jun 04, 2011 |
| Title |
Gene expression analysis of primary human trabecular meshwork cells cultured in fibroblast medium following selective laser trabeculoplasty treatment |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Primary human trabecular meshwork cells cultured in fibroblast medium underwent selective laser trabeculoplasty treatment. RNA was extracted from a pool of cells 30 min after treatment while the remaining cells were further cultured and RNA was extracted respectively 2 and 6 hour after treatment. Control cells stored in incubator in absence of SLT treatment were used as reference samples. Gene expression was evaluated by hybridization on miRNA-microarray and laser scanner analysis.
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| Overall design |
Time course experiment; HTM cells, ScienCell, San Diego, California: cat. n. 6590: isolated from juxtacanalicular and corneoscleral region of the human eye. TMC are cryopreserved on primary culture and delivered frozen.
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| |
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| Contributor(s) |
Izzotti A, Longobardi M, Cartiglia C, Rathschuler F, Saccà SC |
| Citation(s) |
21747927 |
| |
| Submission date |
Jun 02, 2011 |
| Last update date |
Mar 23, 2012 |
| Contact name |
Alberto Izzotti |
| E-mail(s) |
izzotti@unige.it
|
| Phone |
010 3538394
|
| Fax |
010 3538504
|
| Organization name |
University of Genoa
|
| Department |
Dept. of Health Sciences
|
| Street address |
A. Pastore, 1
|
| City |
Genoa |
| ZIP/Postal code |
16132 |
| Country |
Italy |
| |
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| Platforms (1) |
| GPL13679 |
The Microarray Department-University of Amsterdam Human 19K 60mer long oligo array |
|
| Samples (4)
|
| GSM736712 |
primary human trabecular meshwork cells CONTROL |
| GSM736713 |
primary human trabecular meshwork cells T0,5h |
| GSM736714 |
primary human trabecular meshwork cells T2h |
| GSM736715 |
primary human trabecular meshwork cells T6h |
|
| Relations |
| BioProject |
PRJNA141121 |
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