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| Status |
Public on Jan 05, 2012 |
| Title |
Thermotoga species transcription on glucose and a mix of polysaccharides |
| Organisms |
Thermotoga maritima; Thermotoga neapolitana; Thermotoga petrophila; Thermotoga sp. RQ2 |
| Experiment type |
Expression profiling by array
|
| Summary |
Hyperthermophilic bacteria of the genus Thermotoga are known to utilize a wide range of simple and complex polysaccharides. T. maritima's transcriptional response to a variety of mono- and poly-saccharides was previously studied to assign functions to genes involved in carbohydrate uptake and utilization. To compare and contrast closely-related members of the Thermotoga genus, a four-species microarray was developed by expanding a whole genome T. maritima array to include unique genes from three other species (T. neapolitana, T. petrophila, and T. sp. RQ2). This multi-species array was used to investigate the diversity of the genus, specifically the response of each of the four species to a mixture of polysaccharides (galactomannan, glucomannan, xylan, pectin, lichenan, and carboxymethyl cellulose).
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| Overall design |
RNA derived from glucose-grown cultures (glu) was compared to RNA derived from polysaccharide-grown cultures (poly) using a dye swap setup.
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| Contributor(s) |
Frock AD, Gray SR, Kelly RM |
| Citation(s) |
22247137 |
| |
| Submission date |
May 26, 2011 |
| Last update date |
Apr 02, 2012 |
| Contact name |
Andrew Frock |
| E-mail(s) |
adfrock@ncsu.edu
|
| Phone |
9194950054
|
| Organization name |
North Carolina State University
|
| Department |
Chemical and Biomolecular Engineering
|
| Lab |
Hyperthermophile
|
| Street address |
840 Main Campus Drive, Partners II, Room 3304
|
| City |
Raleigh |
| State/province |
NC |
| ZIP/Postal code |
27606 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (4)
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| GSM731853 |
T. maritima polysaccharide mix vs. glucose |
| GSM738020 |
T. neapolitana polysaccharide mix vs. glucose |
| GSM738021 |
T. petrophila polysaccharide mix vs. glucose |
| GSM738022 |
T. sp. RQ2 polysaccharide mix vs. glucose |
|
| Relations |
| BioProject |
PRJNA141325 |
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