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| Status |
Public on Dec 31, 2012 |
| Title |
Gene Expression Profiling of Tuberculous Meningitis coinfected with HIV |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Tuberculosis co-infected with HIV may increase the risk of causing meningitis. Tuberculous meningitis co-infected with HIV associated with high mortality and severe neurological abnormalities in affected individuals. We have carried out TBM co-infected with HIV gene expression study using whole human genome microarrays. We identified 796 differentially expressed genes with fold change cut off of 2 or more than 2. Out of 796 differentially expressed genes, 398 were upregulated and 396 were downregulated. We have validated two molecules from microarray data using immunohistochemistry.
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| Overall design |
The proposed study carried out mRNA expression profiling of five samples from patients diagnosed with tuberculous meningitis coinfected with HIV and four head injury cases were used as controls. We have used 4X44K arrays from agilent platform. To validate our microarray results, we have done immunohistochemistry on 10 TBM+HIV cases and 10 control groups.
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| Contributor(s) |
Pandey A |
| Citation(s) |
27053842 |
| |
| Submission date |
May 24, 2011 |
| Last update date |
Jul 26, 2019 |
| Contact name |
Akhilesh Pandey |
| E-mail(s) |
pandey@jhmi.edu, arivusudar@ibioinformatics.org, sameerkumar.iob@gmail.com, microarray@ibioinformatics.org
|
| Phone |
91 080 28416140
|
| Organization name |
Institute of Bioinformatics
|
| Street address |
UnitI, Discoverer building, ITPL
|
| City |
Bangalore |
| State/province |
Karnataka |
| ZIP/Postal code |
560 066 |
| Country |
India |
| |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (9)
|
| GSM569000 |
Normal_cortex_frozen_tissue_4 |
| GSM730536 |
TBM coinfected with HIV_frozen_tissue 1 |
| GSM730537 |
TBM coinfected with HIV_frozen_tissue 2 |
| GSM730538 |
TBM coinfected with HIV_frozen_tissue 3 |
| GSM730539 |
TBM coinfected with HIV_frozen_tissue 4 |
| GSM730540 |
TBM coinfected with HIV_frozen_tissue 5 |
|
| Relations |
| BioProject |
PRJNA141453 |
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