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Series GSE29253 Query DataSets for GSE29253
Status Public on Mar 01, 2012
Title Gene expression changes upon Dot1L knockdown and Dot1L inhibitor treatment during reprogramming
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Through a loss-of-function approach, we identified that inhibition of the histone methyltransferase, Dot1L, accelerated somatic cell reprogramming, significantly increased the yield of induced pluripotent stem (iPS) cell colonies, and substituted for Klf4 and c-Myc in the reprogramming cocktail. To understand the mechanism by which Dot1L inhibition results in these phenotypes, we carried out gene expression profiling using Affymetrix microarrays.
Overall design GSM723207-GSM723224: Embryonic stem cell-derived fibroblasts (dH1fs) were retrovirally transduced in culture with vectors expressing either a control shRNA or an shRNA targeting Dot1L. These cells were then superinfected with either Oct4, Sox2, Klf4 and c-Myc (OSKM) retroviruses or Oct4, Sox2 and c-Myc (OSM) retroviruses. Total RNA was harvested 6 days later. There were three biologic replicates for each condition.

GSM880675-GSM880682: dH1fs were treated with 10uM Dot1L inhibitor (EPZ004777) and then superinfected with Oct4, Sox2, Klf4 and c-Myc (OSKM) retroviruses. Total RNA was harvested 6 days later. There were two biologic replicates for each condition.
Contributor(s) Onder TT, Daley GQ
Citation(s) 22388813
Submission date May 12, 2011
Last update date Dec 06, 2018
Contact name Tamer T Onder
Organization name Children's Hospital Boston
Street address One Blackfan Circle
City Boston
State/province MA
ZIP/Postal code 02115
Country USA
Platforms (2)
GPL571 [HG-U133A_2] Affymetrix Human Genome U133A 2.0 Array
GPL3921 [HT_HG-U133A] Affymetrix HT Human Genome U133A Array
Samples (26)
GSM723207 dH1f-shCntrl-biorep1
GSM723208 dH1f-shCntrl-biorep2
GSM723209 dH1f-shCntrl-biorep3
BioProject PRJNA139971

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE29253_RAW.tar 59.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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