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| Status |
Public on Feb 02, 2012 |
| Title |
Identification of response genes upon neuronal activation in mouse cortical neurons |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We search for developmental changes specific to humans by examining gene expression profiles in the human, chimpanzee and rhesus macaque prefrontal and cerebellar cortex. In both brain regions, developmental patterns were more evolved in humans than in chimpanzees. The major human specific genes in prefrontal cortex was enriched in neuronal functions and regulated by several transcription factors, which were previously implicated in regulation of neuronal functions. To confirm neuronal function of the human prefrontal cortex specific genes, we identifed response genes upon neuronal activation in mouse cortical neurons. Our results show that human specific genes are enriched in the response genes upon neuronal activation, implying the function of human prefrontal cortex specific genes in synaptic development.
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| Overall design |
The cortical neurons from E15 mouse were isolated and cultured. We then exposed neurons to bicuculline (Bic), or potassium chloride (KCl), or without treatment. The cultured neurons under each group were hybridized to Agilent whole mouse genome oligo microarray (4x44k).
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| Contributor(s) |
Qiu Z, Liu X, Somel M, Khaitovich P |
| Citation(s) |
22300767 |
| Submission date |
May 09, 2011 |
| Last update date |
May 10, 2018 |
| Contact name |
Xiling Liu |
| Organization name |
Partner Institute for Computational Biology
|
| Street address |
320 Yue Yang Road
|
| City |
Shanghai |
| ZIP/Postal code |
200031 |
| Country |
China |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (9)
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| This SubSeries is part of SuperSeries: |
| GSE29140 |
An extreme human-specific delay in cortical synaptic development |
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| Relations |
| BioProject |
PRJNA142873 |
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