 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on Sep 09, 2013 |
| Title |
Toxicogenomic Responses to Inorganic and Organic Mercury in Caenorhabditis elegans |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by array
|
| Summary |
Humans are exposed to both inorganic and organic mercury. While the toxicity of mercury is well established, much remains to be resolved about how different mercurials act at the molecular level. To address this issue, we employed a toxicogenomics approach using the nematode C. elegans. Using sub-, low- or high-toxic exposures of either HgCl2 or CH3HgCl the effects of these mercurials on steady-state mRNA levels for the entire genome were determined. A total of 473 and 2,865 genes were differentially expressed in the HgCl2 and CH3HgCl treatments, respectively. Hierarchical clustering, principal components and pattern analyses indicated that the transcriptional responses of the mercurials were unique.
|
| |
|
| Overall design |
Mixed-stage C. elegans populations were exposed to 0, 2, 7.5 or 20 uM HgCl2 or 0, 0.75, 2, 7.5 uM CH3HgCl for 24 hours. Three independent experiments were performed for each treatment condition.
|
| |
|
| Contributor(s) |
McElwee MK, Freedman JH |
| Citation(s) |
24118919 |
| |
| Submission date |
Apr 28, 2011 |
| Last update date |
Dec 09, 2013 |
| Contact name |
NIEHS Microarray Core |
| E-mail(s) |
microarray@niehs.nih.gov, liuliw@niehs.nih.gov
|
| Organization name |
NIEHS
|
| Department |
DIR
|
| Lab |
Microarray Core
|
| Street address |
111 T.W. Alexander Drive
|
| City |
RTP |
| State/province |
NC |
| ZIP/Postal code |
27709 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL7727 |
Agilent-015061 C. elegans Oligo Microarray 4x44K G2519F (Feature Number version) |
|
| Samples (24)
|
|
| Relations |
| BioProject |
PRJNA140569 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28915_RAW.tar |
163.5 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE28915_tif.tar.gz |
1.3 Gb |
(ftp)(http) |
TAR |
| Processed data included within Sample table |
|
|
|
|
 |
Less...
More...