|
| Status |
Public on Dec 22, 2011 |
| Title |
Son Maintains Accurate Splicing of Pre-mRNAs Encoding Chromatin Modifiers |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Exon and expression analysis of HeLa cells after knockdown of SON
Serine-arginine-rich (SR) proteins play a key role in alternative pre-mRNA splicing in eukaryotes. Our laboratory recently showed that a large SR protein called Son has unique repeat motifs that are essential for maintaining the subnuclear organization of pre-mRNA processing factors in nuclear speckles. Motif analysis of Son highlights putative RNA interaction domains that suggest a direct role for Son in pre-mRNA splicing. A genome-wide screen was performed to identify putative human transcription and splicing targets of Son.
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| |
|
| Overall design |
HeLa cells were transfected with siRNA against SON or a control siRNA (siLuciferase) for 48 hours. Five biological replicates were used for each condition.
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| |
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| Contributor(s) |
Markey MP |
| Citation(s) |
22193954 |
| |
| Submission date |
Apr 17, 2011 |
| Last update date |
Jul 10, 2014 |
| Contact name |
Michael Markey |
| E-mail(s) |
michael.markey@wright.edu
|
| Phone |
937-775-4536
|
| Fax |
937-775-3031
|
| URL |
http://www.med.wright.edu/cgr/
|
| Organization name |
Wright State Unviersity
|
| Department |
Biochemistry and Molecular Biology
|
| Lab |
Center for Genomics Research
|
| Street address |
3640 Colonel Glenn Hwy.
|
| City |
Dayton |
| State/province |
OH |
| ZIP/Postal code |
45435 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL5188 |
[HuEx-1_0-st] Affymetrix Human Exon 1.0 ST Array [probe set (exon) version] |
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| Samples (10)
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| Relations |
| BioProject |
PRJNA138951 |
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