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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Nov 02, 2011 |
| Title |
The tumor suppressor gene rap1GAP is silenced by mir-101-mediated EZH2 overexpression in invasive squamous cell carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Rap1GAP is a critical tumor suppressor gene that is down-regulated in multiple aggressive cancers such as head and neck squamous cell carcinoma, melanoma and pancreatic cancer. However, the mechanistic basis of rap1GAP down-regulation in cancers is poorly understood. By employing an integrative approach, we demonstrate polycomb-mediated repression of rap1GAP that involves EZH2, a histone methyltransferase in head and neck cancers. We further concomitant down-regulation of rap1GAP in head and neck cancers. EZH2 represses rap1GAP by facilitating the trimethylation of H3K27, a mark of gene repression, and also hypermethylation of rap1GAP promoter. These results provide a conceptual framework involving a microRNA-oncogene-tumor suppressor axis to understand head and neck cancer progression.
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| Overall design |
OSCC3-siRNA Non-Targeting Vs. siRNA EZH2 with dye-swap, HOK-Adeno CMV Vs. Adeno EZH2.
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| Contributor(s) |
Banerjee R, Mani R, Russo N, Scanlon CS, Tsodikov A, Jing X, Cao Q, Palanisamy N, Metwally T, Inglehart RC, Tomlins S, Bradford C, Carey T, Wolf G, Kalyana-Sundaram S, Chinnaiyan A, Varambally S, J.D'Silva N |
| Citation(s) |
21532618 |
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| Submission date |
Apr 08, 2011 |
| Last update date |
Feb 22, 2018 |
| Contact name |
NISHA J D'SILVA |
| E-mail(s) |
njdsilva@umich.edu
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| Phone |
734 764 1543
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| Fax |
734 764 2469
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| Organization name |
UNIVERSITY OF MICHIGAN SCHOOL OF DENTISTRY
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| Department |
PERIODONTICS AND ORAL MEDICINE/ SCHOOL OF DENTISTRY
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| Street address |
1011 N. UNIVERSITY AVE, ROOM 5217
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| City |
ANN ARBOR |
| State/province |
MICHIGAN |
| ZIP/Postal code |
48109-1079 |
| Country |
USA |
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| Platforms (1) |
| GPL4133 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Feature Number version) |
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| Samples (3) |
| GSM826835 |
OSCC3-siRNA Non-Targeting (Cy5) Vs. siRNA EZH2 (Cy3) |
| GSM826836 |
OSCC3-siRNA Non-Targeting (Cy3) Vs. siRNA EZH2 (Cy5) |
| GSM826837 |
HOK-Adeno CMV (Cy3) Vs. Adeno EZH2 (Cy5) |
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| Relations |
| BioProject |
PRJNA138989 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28501_RAW.tar |
49.2 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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