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| Status |
Public on May 11, 2013 |
| Title |
Effects of overexpression of Sox2 on gene expression in adult mouse intestine |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
In order to understand the molecular mechanisms of changes seen in the adult mouse intestine in response to Sox2 overexpression, gene expression was analyzed in the ileum upon 2-day Doxycycline (DOX) treatment of mice containing Dox-inducible Sox2 transgene. Repression of Cdx2 and of its intestinal targets was observed and confirmed by Western blotting and immunostaining. This effect of Sox2 was distinct from the effect of overexpression of Oct4.
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| Overall design |
6-8 week old TetON-Sox2, rtTA controls (no TetOP-Sox2 transgene), TetON-Oct4, and TetON-Sox2 X TetON-Oct4 mice (2 replicates) were treated for 2 days with 2 mg/ml DOX or were left untreated. Intestines (ileum part) were collected and total mRNA was extracted.
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| Contributor(s) |
Kuzmichev A, McKay R |
| Citation missing |
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| Submission date |
Apr 07, 2011 |
| Last update date |
May 10, 2018 |
| Contact name |
Andrey Kuzmichev |
| E-mail(s) |
kuzmicha@ninds.nih.gov
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| Organization name |
NINDS/NIH
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| Street address |
35, Lincoln Drive
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| City |
Bethesda |
| State/province |
Maryland |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (10)
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| GSM703384 |
TetON-Sox2 animal +DOX, replica 2 |
| GSM703385 |
TetON-Oct4 animal +DOX, replica 1 |
| GSM703386 |
TetON-Oct4 animal +DOX, replica 2 |
| GSM703387 |
rtTA animal +DOX, replica 1 |
| GSM703388 |
rtTA animal +DOX, replica 2 |
| GSM703389 |
TetON-Oct4 X TetON-Sox2 animal +DOX, replica 1 |
| GSM703390 |
TetON-Oct4 X TetON-Sox2 animal +DOX, replica 2 |
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| Relations |
| BioProject |
PRJNA139203 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28465_RAW.tar |
78.8 Mb |
(http)(custom) |
TAR (of TXT) |
| GSE28465_Sox2+-DOX-normalized.txt.gz |
4.2 Mb |
(ftp)(http) |
TXT |
| GSE28465_rtTA,_Sox2,_Oct4,_OXS-normalized.txt.gz |
5.7 Mb |
(ftp)(http) |
TXT |
| Processed data included within Sample table |
| Processed data are available on Series record |
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