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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Apr 01, 2011 |
| Title |
Seeking Gene Candidates Responsible for Developmental Origins of Health and Disease (DOHaD) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Human epidemiological evidence has led scientists to theorize that undernutrition during gestation is an important early origin of adult diseases. Animal models have successfully demonstrated that maternal diet could contribute to some of adult diseases. Undernutrition is perceived harmful in pregnant women whereas calorie restriction is a strategy proven to extend healthy and maximum life span in adult. This diagrammatically opposite effect of nutritional condition might provide us hints to search for genes underlying health conditions. Here, we have initiated a study examining the effect of undernutrition on maternal and fetal livers, utilizing high-throughput DNA microarray analysis for screening genome-wide changes in their transcriptomes. Briefly, pregnant mice were exposed to food deprivation (FD) on gestation day (GD) 17, and caesarean section was performed on GD18. Control mice were supplied with chow ad libitum till sacrifice. Total RNA extracted from mother and fetal livers for each control and treatment (FD) was analyzed with an Agilent mouse whole genome DNA chip. Total of 3058 and 3126 up (>1.5 fold)- and down (<0.75 fold)-regulated genes, and 1475 and 1225 up- (>1.5 fold)- and down (<0.75 fold)-regulated genes showed differential expression at the mRNA level, in the maternal and fetal livers, respectively. Interestingly, 103 genes up-regulated in mother were down-regulated in fetus, whereas 108 down-regulated maternal genes were up-regulated in fetus; these 211 genes are potential candidates related to longevity or health. The role of some of these genes, in context of the proposed mechanisms for developmental origins of health and disease is discussed.
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| Overall design |
Comparison between mouse control and FD livers in fetus and mother was performed. Five to ten biological replicates were used, and pooled total RNA from each condition (control and FD) was dye-swaped.
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| Contributor(s) |
Ogawa T, Rakwal R, Shibato J, Sawa C, Saito T, Murayama A, Kuwagata M, Kageyama H, Yagi M, Satoh K, Shioda S |
| Citation(s) |
21848995 |
| Submission date |
Mar 25, 2011 |
| Last update date |
May 10, 2018 |
| Contact name |
RANDEEP RAKWAL |
| E-mail(s) |
plantproteomics@gmail.com
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| Phone |
+81-(0)90-1853-7875
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| Organization name |
University of Tsukuba
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| Department |
Institute of Health and Sport Sciences
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| Lab |
GSI 403
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| Street address |
1-1-1 Tennodai
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8574 |
| Country |
Japan |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (4)
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| GSM697640 |
Fetus Control (Cy3) Fetus FD (Cy5) Pooled Sample 1 |
| GSM697641 |
Fetus Control (Cy5) Fetus FD (Cy3) Pooled Sample 2 |
| GSM697645 |
Mother Control (Cy3) Mother FD (Cy5) Pooled Sample 1 |
| GSM697646 |
Mother Control (Cy5) Mother FD (Cy3) Pooled Sample 2 |
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| Relations |
| BioProject |
PRJNA139523 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE28175_RAW.tar |
68.1 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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