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Series GSE27910 Query DataSets for GSE27910
Status Public on Oct 16, 2012
Title Overexpression of NUCKS1 in colorectal cancer correlates with recurrence after curative surgery (copy number analysis)
Organism Homo sapiens
Experiment type Genome variation profiling by SNP array
Summary Purpose: The purpose of this study is to identify a novel biomarker related with distant metastases of colorectal cancer (CRC).
Experimental Design: We investigated mRNA expression profiles in 115 patients with CRC using an Affymetrix Gene Chip, and copy number profiles in 122 patients with CRC using an Affymetrix DNA Sty array. Genes in common between copy number and expression data were extracted as candidate genes. We analyzed the mRNA expression of candidate gene by quantitative reverse transcription polymerase chain reaction (RT-PCR) in 86 patients as a validation study. Furthermore, we analyzed the protein expression of candidate gene by immunohistochemical study in 269 patients, and investigated the relationship between protein expression and clinicopathologic features.
Results: By the combination of copy number analysis and gene expression analysis, We extracted 2 candidate genes related with distant metastases of CRC. Several reports show that NUCKS1, one of candidate genes, is overexpressed in several cancer tissues. But a study about the relationship between NUCKS1 and CRC is none. The mRNA expression of NUCKS1 in cancer tissues was significantly higher than those in normal tissues. Overexpression of NUCKS1 protein was associated with significantly worse relapse-free survival of CRC. Overexpression of NUCKS1 protein was an independent risk factor for recurrence of CRC.
Conclusion: The overexpression of NUCKS1 would be a new biomarker predicting recurrence after colorectal surgery.
 
Overall design Copy number analysis was performed using LCM samples of 122 colorectal cancer patients by GeneChip Human Mapping 250k Sty arrays. Non-tumor tissues obtained from 74 patients were used as unpaired reference samples.
 
Contributor(s) Kikuchi A, Ishikawa T, Iida S, Ishiguro M, Mogushi K, Mizushima H, Uetake H, Tanaka H, Sugihara K
Citation(s) 23065711
Submission date Mar 11, 2011
Last update date Aug 21, 2013
Contact name Kaoru Mogushi
E-mail(s) mogushi-k@umin.ac.jp
Phone +81-3-5802-1797
Organization name Juntendo University
Department Intractable Disease Research Center
Street address 2-1-1 Hongo
City Bunkyo-ku
State/province Tokyo
ZIP/Postal code 113-8421
Country Japan
 
Platforms (1)
GPL3720 [Mapping250K_Sty] Affymetrix Mapping 250K Sty2 SNP Array
Samples (196)
GSM689170 patient 06, cancer, LCM (copy number analysis)
GSM689171 patient 08, cancer, LCM (copy number analysis)
GSM689172 patient 100, cancer, LCM (copy number analysis)
This SubSeries is part of SuperSeries:
GSE27913 Overexpression of NUCKS1 in colorectal cancer correlates with recurrence after curative surgery
Relations
BioProject PRJNA141729

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE27910_RAW.tar 5.0 Gb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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