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| Status |
Public on Nov 21, 2011 |
| Title |
Transcription profile of B cells isolated from peripheral lymph nodes of wild-type vs CCR7-/- mice |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We investigated the transcriptional profile of B cells isolated from peripheral lymph nodes of CCR7-/-C57BL/6 and C57BL/6 mice respectively. B cells were sorted as follows: CD19+, CD3-, CD11c-, CD11b-, NK1.1-, GR-1-. Very few genes were differentially regulated in CCR7-/- B cells, however the expression of a number of genes associated with B-cell activation was increased in CCR7-/- B cells compared to WT B cells. Our data suggest a role of CCR7 signaling in B-cell activation processes.
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| Overall design |
12 purified RNA samples (6 samples of each genotype), originated from 12 individually facs-sorted cell samples, were used for the microarray study. 3 RNA samples per genotype were pooled, giving rise to 2 wildtype- and 2 Ccr7 knock-out pools. In each of the 2 dual-color microarray hybridizations, samples from 1 wildtype and 1 Ccr7 knock-out pool were cohybridized. Microarray hybridizations 1 and 2 were performed in a dye-swap approach.
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| Contributor(s) |
Moschovakis G, Bubke A, Dittrich-Breiholz O, Kremmer E, Förster R |
| Citation(s) |
21969271 |
| Submission date |
Mar 10, 2011 |
| Last update date |
May 10, 2018 |
| Contact name |
Oliver Dittrich-Breiholz |
| E-mail(s) |
dittrich.oliver@mh-hannover.de
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| Organization name |
Medical School Hannover
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| Department |
Research Core Unit Genomics
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| Street address |
Carl-Neuberg-Str. 1
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| City |
Hannover |
| ZIP/Postal code |
30625 |
| Country |
Germany |
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| Platforms (1) |
| GPL4134 |
Agilent-014868 Whole Mouse Genome Microarray 4x44K G4122F (Feature Number version) |
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| Samples (2) |
| GSM688733 |
B cells WT vs CCR7-/- Replicate 1 (Pool1) |
| GSM688734 |
B cells WT vs CCR7-/- Replicate 2 (Pool2) |
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| Relations |
| BioProject |
PRJNA137997 |
