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| Status |
Public on Oct 03, 2024 |
| Title |
The neurotrophic factor MANF regulates autophagic flux and lysosome function to promote proteostasis in C. elegans |
| Organism |
Caenorhabditis elegans |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mesencephalic astrocyte-derived neurotrophic factor (MANF) is the only neurotrophic factor to be conserved between C. elegans and humans. MANF is a unique neutrotophic factor, as it is a resident ER protein in cells that express it and has a role in regulating ER stress response to tunicamycin exposure and pathogenic Pseudomonas exposure. We sought to further understand the role of MANF by comparing a deletion allele, an overexpression line, and the wild-type in this RNAseq experiment.
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| Overall design |
In order to test the role of MANF, we looked at aging animals - we age-synchronized eggs via bleaching gravid adults and grew worms to adulthood. Adult worms were transferred to new plates daily during reproduction in order to separate adults from their offspring. On 8 days post-hatch, we collected worms and isolated RNA, then the Duke Sequencing Core performed poly-A mRNA enrichment and RNA-sequencing.
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| Contributor(s) |
Jessica H, Shane T, Bhagwati G |
| Citation missing |
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| Submission date |
Oct 02, 2024 |
| Last update date |
Oct 04, 2024 |
| Contact name |
Jessica H Hartman |
| E-mail(s) |
hartmanj@musc.edu
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| Phone |
843-876-2308
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| Organization name |
Medical University of South Carolina
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| Department |
Biochemistry and Molecular Biology
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| Street address |
70 President St., Drug Discovery Bldg/Rm DD220
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| City |
Charleston |
| State/province |
South Carolina |
| ZIP/Postal code |
29425 |
| Country |
USA |
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| Platforms (1) |
| GPL18245 |
Illumina HiSeq 2500 (Caenorhabditis elegans) |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA1168187 |
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