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Series GSE27845 Query DataSets for GSE27845
Status Public on Aug 22, 2011
Title TEAD4 regulated genes in differentiated C2C12 myoblasts
Organism Mus musculus
Experiment type Expression profiling by high throughput sequencing
Summary The TEAD (1-4) transcription factors comprise the conserved TEA/ATTS DNA binding domain recognising the MCAT element in the promoters of muscle-specific genes. Despite extensive genetic analysis, the function of TEAD factors in muscle differentiation has proved elusive due to redundancy amongst the family members. Expression of the TEA/ATTS DNA binding domain that acts a dominant negative repressor of TEAD factors in C2C12 myoblasts inhibits their differentiation, while selective shRNA knockdown of TEAD4 results in abnormal differentiation characterised by the formation of shortened myotubes. RNA-seq identifies a novel set of TEAD4 target genes encoding muscle structural and regulatory proteins and those required for the unfolded protein response. In contrast, TEAD4 represses expression of the growth factor CTGF and Cyclin D1 to promote differentiation. Together these results show that TEAD factor activity is essential for normal C2C12 cell differentiation and define a novel and non-redundant role for TEAD4 in regulating expression of the unfolded protein response genes.
Overall design C2C12 cells were infected with lentiviral vectors expressing either a control shRNA or two independent shRNAs directed against TEAD4. The cell populations were selected with puromycin and then differentiated for 7 days to form myotubes.
Contributor(s) Benhaddou A, Keime C, Ye T, Morlon A, Michel I, Jost B, Mengus G, Davidson I
Citation(s) 21701496
Submission date Mar 08, 2011
Last update date Jun 21, 2017
Contact name Celine Keime
Organization name IGBMC (CNRS/INSERM/UDS)
Street address 1 rue Laurent Fries
City Illkirch
ZIP/Postal code 67404
Country France
Platforms (1)
GPL11002 Illumina Genome Analyzer IIx (Mus musculus)
Samples (3)
GSM687322 C2C12_ShSC
GSM687323 C2C12_ShA
GSM687324 C2C12_ShB
BioProject PRJNA138043

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Supplementary file Size Download File type/resource
GSE27845_RAW.tar 211.9 Mb (http)(custom) TAR (of BEDGRAPH)
GSE27845_genes_read_counts.txt.gz 321.3 Kb (ftp)(http) TXT
Processed data provided as supplementary file
Processed data are available on Series record

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