|
| Status |
Public on Sep 30, 2024 |
| Title |
Gene expression profile at single cell level of mesenchymal cells and cancer-associated fibroblasts from mouse pancreas |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Pancreatic stellate cells (PSCs) are components of normal pancreatic mesenchyme, and give rise to cancer-associated fibroblasts during pancreatic tumorigenesis. We used single cell RNA sequencing (scRNA-seq) to analyze PSCs and PSC-derived CAFs from normal pancreas and pancreatic cancer tissues, respectively.
|
| |
|
| Overall design |
PSCs and PSC-derived CAFs were isolated from healthy pancreas tissue and orthotopically implanted pancreatic cancer, respectively, by isolated GFP-positive (normal) and GFP-positive, PDPN-positive (tumor) cells from Rosa26-mTmG;Fabp4-Cre mice.
|
| |
|
| Contributor(s) |
Zheng X, Mara S |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| |
| Submission date |
Sep 26, 2024 |
| Last update date |
Sep 30, 2024 |
| Contact name |
Zheng Xia |
| E-mail(s) |
xiaz@ohsu.edu
|
| Organization name |
Oregon Health Science University
|
| Department |
Department of Biology Medical Engineering
|
| Lab |
Xia Lab
|
| Street address |
2720 S Moody Ave
|
| City |
Portland |
| State/province |
Oregon |
| ZIP/Postal code |
97201 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA1165780 |
