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| Status |
Public on Sep 30, 2024 |
| Title |
Piezo1-mediated Mechanotransduction Shapes ILC2 Translational Activity, Functions, and Lung Pathogenicity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Group-2 innate-lymphoid cells (ILC2s) are critical mediators of the type-2 immune responses in multiple lung pathologies. We show that Piezo1, a mechanosensitive ion channel, plays a key role in regulating ILC2 functions by linking mechanical cues to biochemical signaling pathways. Both murine and human ILC2s strongly express Piezo1, and its activation by Yoda1 selectively enhances IL-13 production through calcium influx, which activates the mTOR-S6K pathway. This pathway leads to translational reprogramming, favoring IL-13 translation. Piezo1-deficient in ILC2s impairs this process, reducing IL-13 levels and resulting in attenuated lung inflammation and fibrosis in mouse models of IL-33- or Alternaria alternata-induced airway inflammation and bleomycin-induced fibrosis. These findings position Piezo1 as a critical mediator of ILC2-driven type-2 immune responses and highlight its potential as a therapeutic target for lung diseases characterized by excessive inflammation. This streamlined understanding of Piezo1 function improves focus on its mechanistic role in lung pathology.
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| Overall design |
mRNA profiles of Yoda1- or DMSO- treated mouse lung ILC2s were generating by bulk mRNA seq using a Nova Seq 6000 (Illumina, Inc., USA).
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| Contributor(s) |
Lim M, Kim H |
| Citation missing |
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| Submission date |
Sep 26, 2024 |
| Last update date |
Oct 01, 2024 |
| Contact name |
MinYeong Lim |
| Organization name |
Seoul National University, College of Medicine
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| Department |
Department of biomedical scicence
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| Street address |
103, Daehak-ro, Jongno-gu
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| City |
Seoul |
| ZIP/Postal code |
03080 |
| Country |
South Korea |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (8)
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| GSM8539395 |
Lung cultured ILC2, DMSO (0.1%), rep4 |
| GSM8539396 |
Lung cultured ILC2, Yoda1 (5µM), rep1 |
| GSM8539397 |
Lung cultured ILC2, Yoda1 (5µM), rep2 |
| GSM8539398 |
Lung cultured ILC2, Yoda1 (5µM), rep3 |
| GSM8539399 |
Lung cultured ILC2, Yoda1 (5µM), rep4 |
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| Relations |
| BioProject |
PRJNA1165661 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE278085_RAW.tar |
780.0 Kb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
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