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| Status |
Public on Sep 30, 2024 |
| Title |
LAG3 regulates the homeostasis of brain white matter microglia and affects astrocyte morphogenesis in the early postnatal period |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
In order to further understand how microglia-derived LAG3 regulates microglia homeostasis and affects the morphogenesis of astrocytes, RNA-seq was used to analyze the transcriptome changes of microglia isolated from Lag3 conditional knockout mice and littermate-born wild-type mice in P7 period. About 7000 transcripts showed the differential expression between the microglia of the corpus callosum of Lag3fl/fl and Lag3cKO-Cx3 mice, with a fold change ≥2, and a p value < 0.05. Geneontology analysis of downregulated genes were enriched in terms related to actin recombination, ciliary recombination and others, while upregulated genes are enriched in inflammatory response and other terms. These results reflected the role of microglia Lag3 in microglia development.
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| Overall design |
mRNA was extracted from microglia isolated from the corpus callosum of Lag3fl/fl and Lag3cKO-Cx3 mice at P7. Specifically, Agilent 2100 Bioanalyze was used to quality controlled and quantified. Then, the mRNA is converted into cDNA and bound the library. RNA-sequencing analysis was carried out using Illumina HiSeq 2500 platform in anno.txtroad Genomics.
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| Contributor(s) |
Li S, Zhang M, Li C, Jiao J |
| Citation missing |
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| Submission date |
Sep 25, 2024 |
| Last update date |
Sep 30, 2024 |
| Contact name |
Jianwei Jiao |
| Organization name |
Institute of Zoology, Chinese Academy of Sciences
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| Department |
State Key Laboratory of Reproductive Biology
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| Street address |
1 Beichen West Road
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| City |
Beijing |
| State/province |
Beijing |
| ZIP/Postal code |
100101 |
| Country |
China |
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| Platforms (1) |
| GPL17021 |
Illumina HiSeq 2500 (Mus musculus) |
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| Samples (4)
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| Relations |
| BioProject |
PRJNA1165146 |
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